Postexercise phosphocreatine recovery, an index of mitochondrial oxidative phosphorylation, is reduced in diabetic patients with lower extremity complications
| Autor: | Chun S. Zuo, Julie Guest, Thanh Dinh, Rosemond A. Villafuerte, Thomas E. Lyons, Christos Sampanis, Charalambos Gnardellis, Aristidis Veves, Francesco Tecilazich |
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| Přispěvatelé: | Tecilazich, Francesco, Dinh, Thanh, Lyons, Thomas E., Guest, Julie, Villafuerte, Rosemond A., Sampanis, Christo, Gnardellis, Charalambo, Zuo, Chun S., Veves, Aristidis |
| Jazyk: | angličtina |
| Předmět: |
Male
Magnetic Resonance Spectroscopy Time Factors Phosphocreatine 030204 cardiovascular system & hematology Mitochondrion Oxidative Phosphorylation chemistry.chemical_compound 0302 clinical medicine Diabetic Neuropathies Granulocyte Colony-Stimulating Factor 80 and over Inflammation Mediator Aged 80 and over Skeletal Middle Aged 3. Good health Peripheral Mitochondria Muscle Female Inflammation Mediators Case-Control Studie Cardiology and Cardiovascular Medicine Type 2 Human Muscle Contraction Adult medicine.medical_specialty Time Factor Diabetic Angiopathie 030209 endocrinology & metabolism Diabetic angiopathy Article 03 medical and health sciences Peripheral Arterial Disease Diabetes mellitus Internal medicine medicine Diabetes Mellitus Humans Muscle Skeletal Exercise Aged business.industry Tumor Necrosis Factor-alpha Case-control study Osteoprotegerin Type 2 Diabetes Mellitus medicine.disease Case-Control Studies Diabetes Mellitus Type 2 Diabetic Angiopathies Surgery Peripheral neuropathy Endocrinology chemistry Diabetic Neuropathie business |
| Zdroj: | Journal of Vascular Surgery. (4):997-1005 |
| ISSN: | 0741-5214 |
| DOI: | 10.1016/j.jvs.2012.10.011 |
| Popis: | To identify differences in postexercise phosphocreatine (PCr) recovery, an index of mitochondrial function, in diabetic patients with and without lower extremity complications. METHODS: We enrolled healthy control subjects and three groups of patients with type 2 diabetes mellitus: without complications, with peripheral neuropathy, and with both peripheral neuropathy and peripheral arterial disease. We used magnetic resonance spectroscopic measurements to perform continuous measurements of phosphorous metabolites (PCr and inorganic phosphate [Pi]) during a 3-minute graded exercise at the level of the posterior calf muscles (gastrocnemius and soleus muscles). Micro- and macrovascular reactivity measurements also were performed. RESULTS: The resting Pi/PCr ratio and PCr at baseline and the maximum reached during exercise were similar in all groups. The postexercise time required for recovery of Pi/PCr ratio and PCr levels to resting levels, an assessment of mitochondrial oxidative phosphorylation, was significantly higher in diabetic patients with neuropathy and those with both neuropathy and peripheral arterial disease (P < .01 for both measurements). These two groups also had higher levels of tumor necrosis factor-α (P < .01) and granulocyte colony-stimulating factor (P < .05). Multiple regression analysis showed that only granulocyte colony-stimulating factor, osteoprotegerin, and tumor necrosis factor-α were significant contributing factors in the variation of the Pi/PCr ratio recovery time. No associations were observed between micro- and macrovascular reactivity measurements and Pi/PCr ratio or PCr recovery time. CONCLUSIONS: Mitochondrial oxidative phosphorylation is impaired only in type 2 diabetes mellitus patients with neuropathy whether or not peripheral arterial disease is present and is associated with the increased proinflammatory state observed in these groups. |
| Databáze: | OpenAIRE |
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