Expression of von Willebrand factor, pulmonary intravascular macrophages, and Toll-like receptors in lungs of septic foals
| Autor: | Leah M. Quanstrom, Alex R. Robinson, Jacqueline M. E. Harrison, Baljit Singh, Stacy L. Anderson, Bruce K. Wobeser |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty 040301 veterinary sciences animal diseases H&E stain Gene Expression Inflammation von Willebrand factor Peripheral blood mononuclear cell digestive system 0403 veterinary science Sepsis 03 medical and health sciences pulmonary intravascular macrophage Von Willebrand factor parasitic diseases Macrophages Alveolar medicine Animals Toll-like receptor 4 and 9 Horses Lung General Veterinary biology business.industry Toll-Like Receptors 04 agricultural and veterinary sciences respiratory system medicine.disease Mononuclear cell infiltration 030104 developmental biology medicine.anatomical_structure immunohistochemistry biology.protein Original Article Horse Diseases medicine.symptom Calprotectin business |
| Zdroj: | Journal of Veterinary Science |
| ISSN: | 1976-555X 1229-845X |
| Popis: | Sepsis causes significant mortality in neonatal foals; however, there is little data describing the cellular and molecular pathways of lung inflammation in septic foals. This study was conducted to characterize lung inflammation in septic foals. Lung tissue sections from control (n = 6) and septic (n = 17) foals were compared using histology and immunohistology. Blinded pathologic scoring of hematoxylin and eosin stained samples revealed increased features of lung inflammation such as thickened alveolar septa and sequestered inflammatory cells in septic foals. Septic foal lungs showed increased expression of von Willebrand factor in blood vessels, demonstrating vascular inflammation. Use of MAC387 antibody to detect calprotectin as a reflection of mononuclear cell infiltration revealed a significant increase in their numbers in alveolar septa of lungs from septic foals compared to those from control foals. The mononuclear cells appeared to be mature macrophages and were located in the septal capillaries, suggesting they were pulmonary intravascular macrophages (PIMs). Finally, lungs from septic foals showed increased expression of Toll-like receptor 4 and 9 in mononuclear cells relative to the control. Taken together, this study is the first to show the expression of inflammatory molecules and an increase in PIMs in lungs from foals that died from sepsis. |
| Databáze: | OpenAIRE |
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