Neurosteroids do not Regulate Proopiomelanocortin-Gene Expression in Pituitary Cells
| Autor: | Florian Holsboer, Walter Zieglgänsberger, F. Parlow, H. Vedder |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
endocrine system
medicine.medical_specialty Pro-Opiomelanocortin Neuroactive steroid Endocrinology Diabetes and Metabolism Gene Expression Dehydroepiandrosterone Pregnanolone Mice chemistry.chemical_compound Endocrinology Glucocorticoid receptor Proopiomelanocortin Corticosterone Internal medicine Internal Medicine medicine Animals RNA Messenger Cells Cultured Brain Chemistry biology Chemistry digestive oral and skin physiology General Medicine Blotting Northern nervous system Pituitary Gland Pregnenolone biology.protein Steroids Corticotropic cell hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Experimental and Clinical Endocrinology & Diabetes. 101:161-165 |
| ISSN: | 1439-3646 0947-7349 |
| DOI: | 10.1055/s-0029-1211224 |
| Popis: | Brain-derived steroids (neurosteroids) constitute a new group of steroids synthesized in brain cells independently from peripheral sources. While the membraneous actions of these steroids have been characterized in some detail, their genomic effects are less well studied up to now. In the present study, the action of the neurosteroids dehydroepiandrosterone, pregnenolone and pregnanolone on proopiomelanocortin (POMC)-gene expression was investigated in pituitary AtT 20 cells, which were cultured under serum-free conditions and at strictly controlled steroid levels. In these cells, POMC-gene expression is known to be negatively regulated by adrenocortical glucocorticoids. Quantitative Northern blot analysis showed that none of these neurosteroids or their conjugates affected POMC-mRNA levels even at the highest concentrations employed. The 21-OH steroid corticosterone was used as a control and markedly suppressed the synthesis of POMC-mRNA. These results suggest that neurosteroids do not control POMC-gene expression at the level of the pituitary corticotroph and exclude significant effects of these compounds at the glucocorticoid receptor. |
| Databáze: | OpenAIRE |
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