DNA interaction with head-to-tail associates of cationic surfactants prevents formation of compact particles
| Autor: | Svetlana Santer, Nina A. Kasyanenko, Ivan Unksov, Vladimir Bakulev |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
head-to-tail surfactant associates
azobenzene trimethylammonium bromide Pharmaceutical Science 02 engineering and technology 010402 general chemistry DNA condensation 01 natural sciences Micelle Article Fluorescence spectroscopy Analytical Chemistry lcsh:QD241-441 Surface-Active Agents chemistry.chemical_compound lcsh:Organic chemistry Pulmonary surfactant Drug Discovery Molecule Physical and Theoretical Chemistry multivalent ions Chemistry Osmolar Concentration Organic Chemistry DNA 021001 nanoscience & nanotechnology 0104 chemical sciences Crystallography Spectrometry Fluorescence Monomer Chemistry (miscellaneous) Ionic strength ddc:540 Institut für Chemie Molecular Medicine Mathematisch-Naturwissenschaftliche Fakultät 0210 nano-technology Azo Compounds Hydrophobic and Hydrophilic Interactions ionic strength |
| Zdroj: | Molecules Volume 23 Issue 7 Molecules, Vol 23, Iss 7, p 1576 (2018) Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry |
| DOI: | 10.25932/publishup-45980 |
| Popis: | Cationic azobenzene-containing surfactants are capable of condensing DNA in solution with formation of nanosized particles that can be employed in gene delivery. The ratio of surfactant/DNA concentration and solution ionic strength determines the result of DNA-surfactant interaction: Complexes with a micelle-like surfactant associates on DNA, which induces DNA shrinkage, DNA precipitation or DNA condensation with the emergence of nanosized particles. UV and fluorescence spectroscopy, low gradient viscometry and flow birefringence methods were employed to investigate DNA-surfactant and surfactant-surfactant interaction at different NaCl concentrations, [NaCl]. It was observed that [NaCl] (or the Debye screening radius) determines the surfactant-surfactant interaction in solutions without DNA. Monomers, micelles and non-micellar associates of azobenzene-containing surfactants with head-to-tail orientation of molecules were distinguished due to the features of their absorption spectra. The novel data enabled us to conclude that exactly the type of associates (together with the concentration of components) determines the result of DNA-surfactant interaction. Predomination of head-to-tail associates at 0.01 M < [NaCl] < 0.5 M induces DNA aggregation and in some cases DNA precipitation. High NaCl concentration (higher than 0.8 M) prevents electrostatic attraction of surfactants to DNA phosphates for complex formation. DAPI dye luminescence in solutions with DNA-surfactant complexes shows that surfactant tails overlap the DNA minor groove. The addition of di- and trivalent metal ions before and after the surfactant binding to DNA indicate that the bound surfactant molecules are located on DNA in islets. |
| Databáze: | OpenAIRE |
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