Expression atlas and comparative coexpression network analyses reveal important genes involved in the formation of lignified cell wall in Brachypodium distachyon

Autor: Sebastian Proost, Staffan Persson, David Roujol, Philippe Le Bris, Laurent Cézart, Richard Sibout, Asher Pasha, Herman Höfte, Frédéric Legée, Federico M. Giorgi, Catherine Lapierre, Bjoern Oest Hansen, Neha Vaid, Oumaya Bouchabke-Coussa, Marek Mutwil, Camille Soulhat, Elisabeth Jamet, Séverine Ho-Yue-Kuang, Nicholas J. Provart
Přispěvatelé: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, Centre for Cancer Genetic Epidemiology [Cambridge], Department of Oncology-University of Cambridge [UK] (CAM), University of Toronto, Laboratoire de Recherche en Sciences Végétales (LRSV), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, University of Melbourne, University of Cambridge [UK] (CAM)-Department of Oncology, Dynamique et Evolution des Parois cellulaires végétales, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Sibout, Richard, Proost, Sebastian, Hansen, Bjoern Oest, Vaid, Neha, Giorgi, Federico M., Ho-Yue-Kuang, Severine, Legée, Frédéric, Cézart, Laurent, Bouchabké-Coussa, Oumaya, Soulhat, Camille, Provart, Nichola, Pasha, Asher, Le Bris, Philippe, Roujol, David, Hofte, Herman, Jamet, Elisabeth, Lapierre, Catherine, Persson, Staffan, Mutwil, Marek
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: New Phytologist
New Phytologist, Wiley, 2017, 215 (3), pp.1009-1025. ⟨10.1111/nph.14635⟩
ISSN: 0028-646X
1469-8137
DOI: 10.1111/nph.14635⟩
Popis: While Brachypodium distachyon (Brachypodium) is an emerging model for grasses, no expression atlas or gene coexpression network is available. Such tools are of high importance to provide insights into the function of Brachypodium genes. We present a detailed Brachypodium expression atlas, capturing gene expression in its major organs at different developmental stages. The data were integrated into a large-scale coexpression database ( www.gene2function.de), enabling identification of duplicated pathways and conserved processes across 10 plant species, thus allowing genome-wide inference of gene function. We highlight the importance of the atlas and the platform through the identification of duplicated cell wall modules, and show that a lignin biosynthesis module is conserved across angiosperms. We identified and functionally characterised a putative ferulate 5-hydroxylase gene through overexpression of it in Brachypodium, which resulted in an increase in lignin syringyl units and reduced lignin content of mature stems, and led to improved saccharification of the stem biomass. Our Brachypodium expression atlas thus provides a powerful resource to reveal functionally related genes, which may advance our understanding of important biological processes in grasses.
Databáze: OpenAIRE
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