Exendin-4 Promotes Beta Cell Proliferation via PI3k/Akt Signalling Pathway
| Autor: | Chengying Gu, Yanju He, Ligang Zhou, Xiaoying Ding, Chaoxun Wang, Xiaopan Chen |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
MAPK/ERK pathway
endocrine system medicine.medical_specialty PI3k/Akt Physiology MAP Kinase Signaling System Incretin lcsh:Physiology lcsh:Biochemistry Mice Phosphatidylinositol 3-Kinases In vivo Internal medicine Insulin-Secreting Cells medicine Animals lcsh:QD415-436 Beta (finance) PI3K/AKT/mTOR pathway Cell Proliferation lcsh:QP1-981 Exendin-4 Akt/PKB signaling pathway Chemistry Venoms Diabetes digestive oral and skin physiology In vitro Cell biology Mice Inbred C57BL Beta-cell proliferation Endocrinology Exenatide Female Beta cell Peptides Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 35, Iss 6, Pp 2223-2232 (2015) |
| ISSN: | 1421-9778 |
| Popis: | Background/Aims: Prevention of diabetes requires maintenance of a functional beta-cell mass, the postnatal growth of which depends on beta cell proliferation. Past studies have shown evidence of an effect of an incretin analogue, Exendin-4, in promoting beta cell proliferation, whereas the underlying molecular mechanisms are not completely understood. Methods: Here we studied the effects of Exendin-4 on beta cell proliferation in vitro and in vivo through analysing BrdU-incorporated beta cells. We also analysed the effects of Exendin-4 on beta cell mass in vivo, and on beta cell number in vitro. Then, we applied specific inhibitors of different signalling pathways and analysed their effects on Exendin-4-induced beta cell proliferation. Results: Exendin-4 increased beta cell proliferation in vitro and in vivo, resulting in significant increases in beta cell mass and beta cell number, respectively. Inhibition of PI3K/Akt signalling, but not inhibition of either ERK/MAPK pathway, or JNK pathway, significantly abolished the effects of Exendin-4 in promoting beta cell proliferation. Conclusion: Exendin-4 promotes beta cell proliferation via PI3k/Akt signaling pathway. |
| Databáze: | OpenAIRE |
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