Primary rat sertoli and interstitial cells exhibit a differential response to cadmium
| Autor: | Stephen R. Clough, Carl D. Brown, Anita H. Payne, Michael J. Welsh, Michael J. Brabec |
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| Rok vydání: | 1990 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Cell type Health Toxicology and Mutagenesis Cell Tetrazolium Salts Cadmium chloride Biology Testicle Tritium Toxicology Interstitial cell Andrology chemistry.chemical_compound Leucine Internal medicine medicine Animals Cells Cultured Sertoli Cells Leydig cell Leydig Cells Proteins Rats Inbred Strains Cell Biology Sertoli cell Rats Fertility medicine.anatomical_structure Endocrinology chemistry Protein Biosynthesis Lactates Oxidation-Reduction Spermatogenesis Cadmium |
| Zdroj: | Cell Biology and Toxicology. 6:63-79 |
| ISSN: | 1573-6822 0742-2091 |
| DOI: | 10.1007/bf00135027 |
| Popis: | Two cell types central to the support of spermatogenesis, the Sertoli cell and the interstitial (Leydig) cell, were isolated from the same cohort of young male rats and challenged with cadmium chloride to compare their susceptibility to the metal. Both cell types were cultured under similar conditions, and similar biochemical endpoints were chosen to minimize experimental variability. These endpoints include the uptake of 109Cd, reduction of the vital tetrazolium dye MTT, incorporation of 3H-leucine, change in heat-stable cadmium binding capacity, and production of lactate. Using these parameters, it was observed that the Sertoli cell cultures were adversely affected in a dose-and time-dependent manner, while the interstitial cell cultures, treated with identical concentrations of CdCl2, were less affected. The 72-hr LC50's for Sertoli cells and interstitial cells were 4.1 and 19.6 microM CdCl2, respectively. Thus, different cell populations within the same tissue may differ markedly in susceptibility to a toxicant. These in vitro data suggest that the Sertoli cell, in relation to the interstitium, is particularly sensitive to cadmium. Because the Sertoli cell provides functional support for the seminiferous epithelium, the differential sensitivity of this cell type may, in part, explain cadmium-induced testicular dysfunction, particularly at doses that leave the vascular epithelium intact. |
| Databáze: | OpenAIRE |
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