Identification of μ-Opioid Receptor Epitopes Recognized by Agonistic IgG
| Autor: | Philippe Druet, E. Druet, Catherine Blanpied, Gilles Dietrich, Gaëtane Macé, Martial Jaume, Christophe Nguyen |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.drug_class Molecular Sequence Data Dose-Response Relationship Immunologic Receptors Opioid mu Biophysics Enzyme-Linked Immunosorbent Assay CHO Cells Ligands Biochemistry Antibodies Epitope Epitopes Antibody Specificity Opioid receptor Cricetinae Cyclic AMP medicine Agonistic behaviour Extracellular Enzyme-linked receptor Animals 5-HT5A receptor Amino Acid Sequence Molecular Biology Sequence Homology Amino Acid biology Chemistry Cell Biology Antigen binding Molecular biology Protein Structure Tertiary Rats Immunoglobulin G biology.protein Antibody Peptides Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 280:1142-1147 |
| ISSN: | 0006-291X |
| Popis: | We have previously reported the presence of IgG antibodies with a morphine-like activity in the serum of healthy individuals. The agonistic activity of IgG was dependent on their binding to the first and the third extracellular loops of the human mu opioid receptor. In this study we show that IgG antibodies obtained by immunizing rats with peptides corresponding to these two loops exhibited a similar morphine-like activity. Residues corresponding to Y(130), M(132), G(133), T(134) within the first and F(315) within the third extracellular segment were required for antibody binding and conferred to IgG a high mu-opioid selectivity. |
| Databáze: | OpenAIRE |
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