Biocompatible Microfabrication of 3D Isolation Chambers for Targeted Confinement of Individual Cells and Their Progeny
| Autor: | Bryan Kaehr, C. Jeffrey Brinker, Jason C. Harper, Susan M. Brozik |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Staphylococcus aureus
Cell Survival Chemistry technology industry and agriculture High density Biocompatible Materials Hydrogels Serum Albumin Bovine Nanotechnology Cell Separation Saccharomyces cerevisiae Biocompatible material Multiphoton lithography Isolation (microbiology) Analytical Chemistry Self-healing hydrogels Escherichia coli Animals Microtechnology Microfabrication |
| Zdroj: | Analytical Chemistry. 84:8985-8989 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac301816c |
| Popis: | We describe a technique to physically isolate single/individual cells from their surrounding environment by fabricating three-dimensional microchambers around selected cells under biocompatible conditions. Isolation of targeted cells is achieved via rapid fabrication of protein hydrogels from a biocompatible precursor solution using multiphoton lithography, an intrinsically 3D laser direct write microfabrication technique. Cells remain chemically accessible to environmental cues enabling their propagation into well-defined, high density populations. We demonstrate this methodology on gram negative (E. coli), gram positive (S. aureus), and eukaryotic (S. cerevisiae) cells. The opportunities to confine viable, single/individual-cells and small populations within user-defined microenvironments afforded by this approach should facilitate the study of cell behaviors across multiple generations. |
| Databáze: | OpenAIRE |
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