Biochemical and gene expression analyses of conotoxins in Conus textile venom ducts
| Autor: | Maren Watkins, Olga Buczek, James E. Garrett, Baldomero M. Olivera, Grzegorz Bulaj |
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| Rok vydání: | 2005 |
| Předmět: |
Conus textile
Molecular Sequence Data Snails Biophysics Venom complex mixtures Biochemistry Conus Animals Gene family Tissue Distribution Amino Acid Sequence Conotoxin Molecular Biology Regulation of gene expression biology Gene Expression Profiling Cell Biology biology.organism_classification Molecular biology Molecular Weight Gene expression profiling Gene Expression Regulation Organ Specificity Conus Snail Conotoxins |
| Zdroj: | Biochemical and Biophysical Research Communications. 328:362-367 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2004.12.178 |
| Popis: | Each Conus snail species produces 50-200 unique peptide-based conotoxins, derived from a number of different gene superfamilies. Conotoxins are synthesized and secreted in a long venom duct, but biochemical and molecular aspects of their biosynthesis remain poorly understood. Here, we analyzed expression patterns of conotoxin genes belonging to different superfamilies in Conus textile venom ducts. The results demonstrate that specific gene families are expressed in particular regions of the venom duct. Biochemical analysis using liquid chromatography and mass spectrometry revealed an even more localized accumulation of individual conotoxins. This study demonstrates for the first time that specialization of gene expression, processing, and secretion of conotoxins occurs in different regions of the venom duct. |
| Databáze: | OpenAIRE |
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