Complete Genomic Sequence of Bacteriophage φEcoM-GJ1, a Novel Phage That Has Myovirus Morphology and a Podovirus-Like RNA Polymerase
| Autor: | Andrew M. Kropinski, Josée Harel, Nidham Jamalludeen, Erika J. Lingohr, Carlton L. Gyles, Roger P. Johnson |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
viruses
Molecular Sequence Data Genetics and Molecular Biology Myoviridae Genome Viral Polymerase Chain Reaction Applied Microbiology and Biotechnology Genome Bacteriophage Siphoviridae Podoviridae Enterotoxigenic Escherichia coli Bacteriophages Gene Genetics Base Composition Base Sequence Ecology biology Intron Computational Biology DNA-Directed RNA Polymerases Sequence Analysis DNA Potyviridae biology.organism_classification Molecular biology Microscopy Electron Lytic cycle Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 74:516-525 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.00990-07 |
| Popis: | The complete genome of φEcoM-GJ1, a lytic phage that attacks porcine enterotoxigenic Escherichia coli of serotype O149:H10:F4, was sequenced and analyzed. The morphology of the phage and the identity of the structural proteins were also determined. The genome consisted of 52,975 bp with a G+C content of 44% and was terminally redundant and circularly permuted. Seventy-five potential open reading frames (ORFs) were identified and annotated, but only 29 possessed homologs. The proteins of five ORFs showed homology with proteins of phages of the family Myoviridae , nine with proteins of phages of the family Podoviridae , and six with proteins of phages of the family Siphoviridae . ORF 1 encoded a T7-like single-subunit RNA polymerase and was preceded by a putative E. coli σ 70 -like promoter. Nine putative phage promoters were detected throughout the genome. The genome included a tRNA gene of 95 bp that had a putative 18-bp intron. The phage morphology was typical of phages of the family Myoviridae , with an icosahedral head, a neck, and a long contractile tail with tail fibers. The analysis shows that φEcoM-GJ1 is unique, having the morphology of the Myoviridae , a gene for RNA polymerase, which is characteristic of phages of the T7 group of the Podoviridae , and several genes that encode proteins with homology to proteins of phages of the family Siphoviridae . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|