Decreased follicular steroids and insulin-like growth factor-I and increased atresia in diabetic gilts during follicular growth stimulated with PMSG
| Autor: | R. C. Tubbs, N. M. Cox, I. A. Matamoros, K. A. Meurer |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Blood Glucose
Embryology medicine.medical_specialty Gonadotropins Equine Swine medicine.medical_treatment Follicular Atresia Ovary Biology Diabetes Mellitus Experimental Insulin-like growth factor Endocrinology Ovarian Follicle Diabetes mellitus Internal medicine Follicular phase medicine Animals Testosterone Ovarian follicle Insulin-Like Growth Factor I Gonadal Steroid Hormones Progesterone Estradiol Follicular atresia Insulin Obstetrics and Gynecology Cell Biology Luteinizing Hormone medicine.disease Follicular fluid Follicular Fluid medicine.anatomical_structure Reproductive Medicine Female Follicle Stimulating Hormone |
| Zdroj: | Journal of reproduction and fertility. 91(1) |
| ISSN: | 0022-4251 |
| Popis: | Four streptozotocin-diabetic gilts (maintained on exogenous insulin for 3 months) and 4 normoglycaemic gilts were treated with 600 i.u. PMSG. Diabetic gilts had insulin therapy removed at the time of PMSG administration. Plasma glucose averaged 463 +/- 5 mg/100 ml for diabetic gilts and 82 +/- 4 mg/100 ml for control gilts over the 72-h sampling period. Serum insulin was lower in diabetic than in normoglycaemic gilts (glycaemic state by time interaction; P less than 0.0001). At ovary removal 75 h after PMSG, numbers and percentages of large (greater than or equal to 7 mm) and medium (3-6 mm) non-atretic follicles were similar for diabetic and control gilts (31 vs 68%; s.e.m. = 7; P less than 0.05). Diabetic gilts had a greater percentage of atretic follicles over all size classes (50 vs 21%; s.e.m. = 7; P less than 0.03). After PMSG, LH was suppressed within 12 h in control gilts and remained similar to values in diabetic gilts until 72 h, when LH was elevated in 2 diabetic gilts (glycaemic state by time interaction; P less than 0.001). Pulsatile LH patterns during 52-55 h after PMSG were not affected by glycaemic state. Serum concentrations of IGF-I tended (P less than 0.1) to be lower in diabetic gilts. Concentrations of oestradiol and FSH in serum were similar in diabetic and control gilts. Follicular fluid concentrations of oestradiol in follicles greater than or equal to 7 mm were lower in diabetic than normoglycaemic gilts (341 vs 873 ng/ml; s.e.m. = 86; P less than 0.05). Testosterone was higher in follicles 3-6 mm in diameter in diabetic than in normoglycaemic gilts (142 vs 80 ng/ml; s.e.m. = 26; P less than 0.05). Progesterone concentrations in follicular fluid were not affected by glycaemic state. Concentrations of IGF-I in follicles greater than or equal to 7 mm were lower in diabetic than control gilts (150 vs 200 ng/ml; s.e.m. = 13; P less than 0.05). We conclude that follicles of diabetic gilts respond to external gonadotrophic stimulation with decreased hormone production and increased ovarian follicular atresia, despite an absence of effects on circulating gonadotrophin and oestradiol concentrations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|