Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits
Autor: | Věra Lukášová, Eva Filová, Jiří Beznoska, Jiří Chvojka, Martin Plencner, Zbyněk Tonar, Andrea Staffa, Anna Malečková, Andrej Litvinec, Evžen Amler, Matěj Buzgo, Miroslav Soural, Jana Daňková, Milena Králíčková, Michala Rampichová |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
collagen
Biocompatibility General Chemical Engineering 02 engineering and technology macromolecular substances Bone tissue complex mixtures Article lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Hyaluronic acid medicine poly-ε-caprolactone General Materials Science fibrin cartilage 030304 developmental biology microparticles 0303 health sciences Hyaline cartilage Cartilage fungi technology industry and agriculture 021001 nanoscience & nanotechnology Chondrogenesis musculoskeletal system equipment and supplies medicine.anatomical_structure chemistry lcsh:QD1-999 Nanofiber Self-healing hydrogels CD44 antibody 0210 nano-technology Biomedical engineering |
Zdroj: | Nanomaterials Volume 10 Issue 8 Nanomaterials, Vol 10, Iss 1504, p 1504 (2020) |
ISSN: | 2079-4991 |
DOI: | 10.3390/nano10081504 |
Popis: | Hydrogels are suitable for osteochondral defect regeneration as they mimic the viscoelastic environment of cartilage. However, their biomechanical properties are not sufficient to withstand high mechanical forces. Therefore, we have prepared electrospun poly-&epsilon caprolactone-chitosan (PCL-chit) and poly(ethylene oxide)-chitosan (PEO-chit) nanofibers, and FTIR analysis confirmed successful blending of chitosan with other polymers. The biocompatibility of PCL-chit and PEO-chit scaffolds was tested fibrochondrocytes and chondrocytes seeded on PCL-chit showed superior metabolic activity. The PCL-chit nanofibers were cryogenically grinded into microparticles (mean size of about 500 µ m) and further modified by polyethylene glycol&ndash biotin in order to bind the anti-CD44 antibody, a glycoprotein interacting with hyaluronic acid (PCL-chit-PEGb-antiCD44). The PCL-chit or PCL-chit-PEGb-antiCD44 microparticles were mixed with a composite gel (collagen/fibrin/platelet rich plasma) to improve its biomechanical properties. The storage modulus was higher in the composite gel with microparticles compared to fibrin. The Eloss of the composite gel and fibrin was higher than that of the composite gel with microparticles. The composite gel either with or without microparticles was further tested in vivo in a model of osteochondral defects in rabbits. PCL-chit-PEGb-antiCD44 significantly enhanced osteogenic regeneration, mainly by desmogenous ossification, but decreased chondrogenic differentiation in the defects. PCL-chit-PEGb showed a more homogeneous distribution of hyaline cartilage and enhanced hyaline cartilage differentiation. |
Databáze: | OpenAIRE |
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