The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts

Autor: Patrícia Pinto Saraiva, Bianca Mariani Gonçalves, Regiane Marques Castro Olimpio, Bruna Moretto Rodrigues, Miriane de Oliveira, Lucas Solla Mathias, Maria Teresa De Sibio, Célia Regina Nogueira, Igor Carvalho Deprá, Durvanei Augusto Maria, Fernanda Cristina Fontes Moretto, Helena Paim Tilli
Přispěvatelé: Universidade Estadual Paulista (Unesp), Butantan Institute
Rok vydání: 2019
Předmět:
0301 basic medicine
musculoskeletal diseases
medicine.medical_specialty
Osteoclasts
Stem cells
030226 pharmacology & pharmacy
Hyperthyroidism
General Biochemistry
Genetics and Molecular Biology
Bone and Bones
Bone remodeling
03 medical and health sciences
0302 clinical medicine
Osteoprotegerin
Osteogenesis
Internal medicine
Gene expression
medicine
Humans
General Pharmacology
Toxicology and Pharmaceutics
Triiodothyronine
Osteoblasts
biology
Receptor Activator of Nuclear Factor-kappa B
Chemistry
Osteoblast
RANK Ligand
RANKL and OPG
Cell Differentiation
Estrogens
Mesenchymal Stem Cells
General Medicine
Alkaline Phosphatase
Estrogen
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Gene Expression Regulation
RANKL
Osteocalcin
biology.protein
Alkaline phosphatase
Bone Remodeling
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1879-0631
Popis: Made available in DSpace on 2019-10-06T16:34:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-08-15 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Triiodothyronine (T3) and estrogen (E2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa β (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism and associated E2 in human cells. AIMS: This study evaluated the effects of the physiological concentration of E2 (10 nM), alone or in association with physiological (1 nM) and supraphysiological (10 nM) concentrations of T3, on RANKL and OPG gene expression in human osteoblasts. MAIN METHODS: Alkaline phosphatase and osteocalcin assays were performed to verify the presence of mature osteoblasts. After mimicking the experimental hyperthyroidism in osteoblasts untreated or treated with E2, RANKL and OPG gene expression was analyzed by real-time PCR and protein expression by western Blot and ELISA. Alizarin Red staining analyzed the amount of bone matrix after hormonal treatments. KEY FINDINGS: E2 enhanced the gene expression of OPG when associated with 1 nM and 10 nM T3. E2 was able to restore the bone matrix after an initial decrease using 1 nM and 10 nM T3. The protective effect of E2 on the RANKL and OPG signaling pathway was demonstrated. E2 restored the bone matrix induced by experimental hyperthyroidism. SIGNIFICANCE: The data highlight the importance of E2 to maintain OPG expression and osteoblast activity against possible loss of bone mass, especially in conditions where T3 is in excess. Department of Internal Medicine Botucatu Medical School Univ Estadual Paulista - UNESP Laboratory Biochemistry and Biophysics Butantan Institute, 1500, Avenue Vital Brazil Department of Internal Medicine Botucatu Medical School Univ Estadual Paulista - UNESP FAPESP: 2014/16406-9
Databáze: OpenAIRE
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