Light-up fluorophore--DNA aptamer pair for label-free turn-on aptamer sensors
| Autor: | Ryota Kimura, Teru Kato, Ippei Shimada, Masumi Hyuga |
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| Rok vydání: | 2016 |
| Předmět: |
Analyte
Fluorophore Light Aptamer Biosensing Techniques 010402 general chemistry 01 natural sciences Catalysis Fluorescence Turn (biochemistry) chemistry.chemical_compound Materials Chemistry Label free 010405 organic chemistry Chemistry Metals and Alloys General Chemistry DNA Aptamers Nucleotide 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Spectrometry Fluorescence Ceramics and Composites Biophysics Light Up |
| Zdroj: | Chemical communications (Cambridge, England). 52(21) |
| ISSN: | 1364-548X |
| Popis: | We developed a light-up fluorophore-DNA aptamer pair for label-free aptamer sensors that fluoresce upon binding to the analyte. A 42mer DNA aptamer binding to the environment-sensitive fluorophore, dapoxyl, which increased the fluorescence by more than 700-fold upon binding, was successfully used to construct aptamer sensors by fusion with analyte-binding DNA aptamers. |
| Databáze: | OpenAIRE |
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