Approaches to functional genomics: potential of matrix-assisted laser desorption ionization--time of flight mass spectrometry combined with separation methods for the analysis of DNA in biological samples
| Autor: | William S. Hancock, Yuqin Dai, Alex Apffel, Liang Li, Karen M. Hahnenberger, Kay Lichtenwalter, John A Chakel, Jian Bai |
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| Rok vydání: | 2000 |
| Předmět: |
Gel electrophoresis
Chromatography Genome Base pair Oligonucleotide Analytical chemistry General Chemistry DNA Picolinic acid Mass spectrometry chemistry.chemical_compound Restriction map Plasmid chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 745(1) |
| ISSN: | 1387-2273 |
| Popis: | MALDI-TOF MS has potential as a valuable technique in DNA mapping studies and may well be complementary to other approaches to DNA analysis such as gel electrophoresis and sequencing. This study used 2,6-dihydroxyacetophenone (DHAP) mixed with diammonium hydrogen citrate (DAHC) as the matrix. In addition, recent technical advances such as time lag focussing (TLF) and better selection of matrices (such as 3-hydroxypicolinic acid (3 HPA) and picolinic acid (PA)) extended the range of DNA fragments that can be studied by this approach. The following samples were investigated: Poly-T mixture (dT 15, 19, 20, 25, 74 and 75), plasmid pBR322 derived oligonucleotides (10, 11, 12, 13, 14, 15, 19, 20 and 50 nucleotides long) and DNA fragments of 25, 36 and 37 base pairs corresponding to a fragment in the restriction map for the gene corresponding to the hexon protein of Adenovirus 2 and 5. The results were contrasted with similar analyses performed by ion-paired reversed-phase HPLC coupled to on-line electrospray mass spectrometry. |
| Databáze: | OpenAIRE |
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