Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers
| Autor: | Fengbin Wang, Cong Shen, Yangqi Gu, Nicole L. Ing, Nikhil S. Malvankar, Allon I. Hochbaum, Dennis Vu, J. Patrick O'Brien, Edward H. Egelman, Sophia M. Yi, Sibel Ebru Yalcin, Vishok Srikanth |
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| Rok vydání: | 2018 |
| Předmět: |
cytochromes
Cytochrome cryoelectron microscopy Nanowire Electrons Heme bioelectronics Medical and Health Sciences General Biochemistry Genetics and Molecular Biology Article Fimbriae Protein filament Electron Transport 03 medical and health sciences 0302 clinical medicine Protein structure protein structure electron conductivity Geobacter sulfurreducens 030304 developmental biology chemistry.chemical_classification 0303 health sciences atomic force microscopy biology Nanowires Bacterial Electric Conductivity Biological Sciences Electron acceptor biology.organism_classification Electron transport chain microbial nanowires chemistry Chemical physics Biofilms Fimbriae Bacterial biology.protein extracellular electron transport Fimbriae Proteins Geobacter Oxidation-Reduction 030217 neurology & neurosurgery biomaterials Developmental Biology |
| Zdroj: | Wang, Fengbin; Gu, Yangqi; O’Brien, J Patrick; Yi, Sophia M; Yalcin, Sibel Ebru; Srikanth, Vishok; et al.(2019). Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers. Cell, 177(2), 361-369.e10. doi: 10.1016/j.cell.2019.03.029. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/1112h2x2 Cell, vol 177, iss 2 |
| ISSN: | 1097-4172 |
| Popis: | © 2019 Elsevier Inc. Long-range (>10 μm) transport of electrons along networks of Geobacter sulfurreducens protein filaments, known as microbial nanowires, has been invoked to explain a wide range of globally important redox phenomena. These nanowires were previously thought to be type IV pili composed of PilA protein. Here, we report a 3.7 Å resolution cryoelectron microscopy structure, which surprisingly reveals that, rather than PilA, G. sulfurreducens nanowires are assembled by micrometer-long polymerization of the hexaheme cytochrome OmcS, with hemes packed within ∼3.5–6 Å of each other. The inter-subunit interfaces show unique structural elements such as inter-subunit parallel-stacked hemes and axial coordination of heme by histidines from neighboring subunits. Wild-type OmcS filaments show 100-fold greater conductivity than other filaments from a ΔomcS strain, highlighting the importance of OmcS to conductivity in these nanowires. This structure explains the remarkable capacity of soil bacteria to transport electrons to remote electron acceptors for respiration and energy sharing. Stacked heme filaments form the structural basis for long-range electron transport in bacterial nanowires. |
| Databáze: | OpenAIRE |
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