Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

Autor: Christopher K. Cote, David P Fetterer, Matthew C. Reed, Sharon P. Daye, Sergei S. Biryukov, Steven J. Kern, Kristen A. Fritts, Kei Amemiya, Ronald G. Toothman, Melissa G. Hunter, Christopher P. Klimko, Carl Soffler, Avery V. Quirk, Susan L. Welkos, Jennifer L. Shoe, Joel A. Bozue, Sylvia R. Trevino, Norman Kreiselmeier, Lara J. Kohler, Jennifer L. Dankmeyer, Michael J. Aponte-Cuadrado, Patricia L. Worsham, Joshua R. Meyer, Christopher W. Schellhase, David M. Waag
Rok vydání: 2018
Předmět:
0301 basic medicine
Bacterial Diseases
Melioidosis
Burkholderia pseudomallei
Physiology
Disease
Pathology and Laboratory Medicine
Pathogenesis
White Blood Cells
0302 clinical medicine
Animal Cells
Immune Physiology
Medicine and Health Sciences
Immune Response
Materials
Mice
Inbred BALB C
Innate Immune System
Multidisciplinary
biology
Virulence
T Cells
Animal Models
Thailand
medicine.anatomical_structure
Infectious Diseases
Experimental Organism Systems
Physical Sciences
Disease Progression
Medicine
Cytokines
Female
Cellular Types
Research Article
Science
Immune Cells
Immunology
Materials Science
Spleen
Bronchi
Mouse Models
Research and Analysis Methods
Microbiology
03 medical and health sciences
Immune system
Signs and Symptoms
Model Organisms
Diagnostic Medicine
medicine
Animals
Humans
Inflammation
Aerosols
Blood Cells
Australia
Biology and Life Sciences
Cell Biology
Molecular Development
medicine.disease
biology.organism_classification
Mice
Inbred C57BL
Disease Models
Animal
030104 developmental biology
Immunoglobulin G
Immune System
Mixtures
Antibody Formation
Animal Studies
Bacteria
030215 immunology
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 11, p e0208277 (2018)
ISSN: 1932-6203
Popis: Mouse models have been essential to generate supporting data for the research of infectious diseases. Burkholderia pseudomallei, the etiological agent of melioidosis, has been studied using mouse models to investigate pathogenesis and efficacy of novel medical countermeasures to include both vaccines and therapeutics. Previous characterization of mouse models of melioidosis have demonstrated that BALB/c mice present with an acute infection, whereas C57BL/6 mice have shown a tendency to be more resistant to infection and may model chronic disease. In this study, either BALB/c or C57BL/6 mice were exposed to aerosolized human clinical isolates of B. pseudomallei. The bacterial strains included HBPUB10134a (virulent isolate from Thailand), MSHR5855 (virulent isolate from Australia), and 1106a (relatively attenuated isolate from Thailand). The LD50 values were calculated and serial sample collections were performed in order to examine the bacterial burdens in tissues, histopathological features of disease, and the immune response mounted by the mice after exposure to aerosolized B. pseudomallei. These data will be important when utilizing these models for testing novel medical countermeasures. Additionally, by comparing highly virulent strains with attenuated isolates, we hope to better understand the complex disease pathogenesis associated with this bacterium.
Databáze: OpenAIRE
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