Transcriptomic analysis of differential gene expression reveals an increase in COX2 levels during in vitro canine herpesvirus infection
| Autor: | João Pessoa Araújo Júnior, Jacqueline Kazue Kurissio |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
herpesvirus CaHV-I Viral pathogenesis Virus lcsh:Agriculture Transcriptome 03 medical and health sciences Canine herpesvirus herpesvirus Gene expression lcsh:Agriculture (General) Gene Pathogen CaHV-1 General Veterinary biology canine pathogen PTGS2 lcsh:S food and beverages biology.organism_classification lcsh:S1-972 Molecular biology 030104 developmental biology Viral replication Animal Science and Zoology Agronomy and Crop Science transcriptome COX2 |
| Zdroj: | Ciência Rural v.48 n.10 2018 Ciência Rural Universidade Federal de Santa Maria (UFSM) instacron:UFSM Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Ciência Rural, Vol 48, Iss 10 (2018) |
| Popis: | EnglishCanine herpesvirus (CaHV-1) affects canids worldwide, causing death in neonates and immunosuppressed hosts. Acute infection by CaHV-1 can cause reproductive, respiratory, and neurological problems in adult animals. Viral pathogenesis and host genes expressions during of CaHV-1infection are not clearly understood. In the present study, the transcriptome of canine kidney cell Mardin-Darby (MDCK) infected in vitro with canine herpesvirus was explored. For this, RNA sequencing (RNA-seq) of the samples in different moments during infection was carried out. Subsequently, the transcriptomic analysis genes related to cell activities and process involved to viral cycle infection were evaluated until 32h post-inoculation (pi). Among evaluated genes, was verified a significant and gradative increase of the prostaglandin-endoperoxide synthase 2 (PTGS2) or cyclooxygenase 2 (COX2) gene expression, throughout of infection, though differential gene expression analysis and validated by quantitative reverse transcription PCR (RT-qPCR). High COX2 expression is usually induced in response to inflammation, pathogens or activation of the immune system but can be a viral mechanism to favor viral replication. Thus, COX2 level increase can be a favorable factor for viral infection with Cahv-1 virus and the use of selective COX2 inhibitors may be beneficial for limiting the infection or clinical signs by causing interruption of the viral replication cycle during active infection. Additionally, the regulation genes expression differential verified in this study can contribute to determining important targets for inhibiting canine herpesvirus infection either by cellular or viral mechanisms. Key words: transcriptome; herpesvirus; CaHV-1; PTGS2; COX2; canine pathogen portuguesO herpesvirus canino (CaHV-1) afeta os canideos em todo o mundo, causando morte em neonatos e hospedeiros imunossuprimidos. A infeccao aguda por CaHV-1 pode causar problemas reprodutivos, respiratorios e neurologicos em animais adultos. A patogenese viral e expressao de genes hospedeiros durante a infeccao por CaHV-1 ainda nao sao bem compreendidos. No presente estudo, o transcriptoma de celulas de rim canino Madin-Darby (MDCK) infectadas in vitro com herpesvirus canino foi explorado. Para isso, foi realizado o sequenciamento de RNA (RNA-seq) de amostras coletadas em diferentes momentos durante a infeccao. Subsequentemente, a analise transcriptomica dos genes relacionados a atividade celular e aos processos envolvidos no ciclo de infeccao do virus foram avaliadas ate 32 horas apos a inoculacao (pi). Dentre os genes avaliados, constatamos uma elevacao significativa e gradativa da expressao da Prostaglandina-endoperoxide sintase 2 (PTGS2) ou ciclooxigenase 2 (COX2), ao longo da infeccao viral, foi verificada por analise de expressao genica diferencial e validada por resultados de transcricao reversa por PCR quantitativo (RT-qPCR). O aumento da expressao de COX2 geralmente e induzida em resposta a inflamacao, patogenos ou ativacao do sistema imune, mas tambem pode ser um mecanismo para favorecer a replicacao viral. Assim, o aumento do nivel de COX2 pode ser um fator favoravel a infeccao viral pelo virus CaHV-1 e o uso de inibidores seletivos da COX2 pode ser benefico para limitar a infeccao ou os sinais clinicos, causando a interrupcao do ciclo de replicacao viral durante a infeccao ativa. Alem disso, a regulacao diferencial da expressao dos genes verificados neste estudo podem contribuir para determinar alvos importantes para inibir a infeccao por herpesvirus canino, seja por mecanismos celulares ou virais. Palavras-chave: transcriptome; RNA-seq; herpesvirus; CaHV-1; COX2; patogeno |
| Databáze: | OpenAIRE |
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