BOVINE CYTOTOXIC T-CELL CLONES SPECIFIC FOR CELLS INFECTED WITH THE PROTOZOAN PARASITE THEILERIA-PARVA - PARASITE STRAIN SPECIFICITY AND CLASS-I MAJOR HISTOCOMPATIBILITY COMPLEX RESTRICTION
| Autor: | Albert Bensaid, Wi Morrison, Cynthia L. Baldwin, Bruno Goddeeris, Alan J. Teale |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1986 |
| Předmět: |
medicine.drug_class
Theileria parva Antigens Protozoan Theileriasis/immunology Major histocompatibility complex Monoclonal antibody Antigen Histocompatibility Antigens parasitic diseases medicine Cytotoxic T cell Animals Antibodies Monoclonal/immunology Multidisciplinary biology Clone Cells/immunology Apicomplexa/immunology Antibodies Monoclonal MHC restriction biology.organism_classification T-Lymphocytes Cytotoxic/immunology Virology Clone Cells Theileriasis Histocompatibility Antigens/immunology Antigens Surface/immunology Antigens Surface biology.protein Antigens Protozoan/immunology Cattle Immunization Antibody Apicomplexa CD8 T-Lymphocytes Cytotoxic Research Article |
| Zdroj: | Goddeeris, B, Morrison, I, Teale, A J, Bensaid, A & Baldwin, C L 1986, ' Bovine cytotoxic T-cell clones specific for cells infected with the protozoan parasite Theileria parva: parasite strain specificity and class I major histocompatibility complex restriction ', Proceedings of the National Academy of Sciences, vol. 83, no. 14, pp. 5238-42 . |
| Popis: | We present information on the specificity of three bovine cytotoxic T-cell clones reactive with lymphoblasts infected with the protozoan parasite Theileria parva. The clones were derived from peripheral blood mononuclear cells of an animal immunized with T. parva (Muguga stock), after five stimulations in vitro with an autologous parasitized cell line. The three clones belonged to the BoT8+ subset of T cells, which is similar to the human CD8+ T-cell subset. On the basis of analysis on a panel of infected target cells originating from cattle of different major histocompatibility complex (MHC) phenotypes, killing by all three clones was found to be restricted to targets bearing the class I MHC specificity KN104, which is defined by alloantiserum KNA104 and monoclonal antibody IL-A4. This class I MHC restriction was confirmed by blocking of target cell lysis with these antibodies and monoclonal antibody w6/32, which reacts with a nonpolymorphic determinant on bovine class I MHC molecules. The three clones were parasite strain specific, in that they did not kill cells of the appropriate MHC type infected with T. parva (Marikebuni stock). These findings, taken together with previous observations that immunization of cattle with T. parva (Muguga) does not provide protection against challenge with T. parva (Marikebuni), suggest that the cytotoxic T cells recognize a cell surface antigen that may be important in induction of immunity to the parasite. ispartof: Proceedings of the National Academy of Sciences of the United States of America vol:83 issue:14 pages:5238-5242 ispartof: location:United States status: published |
| Databáze: | OpenAIRE |
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