Severe dietary magnesium deficiency does not alter levels and function of myocardial Gs alpha and Gi alpha
| Autor: | J. E. Heavner, B. Shi, J. E. Spallholz, L. M. Boylan |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Physiology G protein Heart Ventricles Arbitrary unit Immunoblotting Alpha (ethology) chemistry.chemical_element GTP-Binding Protein alpha Subunits Gi-Go Biology Hypomagnesemia Rats Sprague-Dawley Adenylyl cyclase chemistry.chemical_compound Physiology (medical) Internal medicine Magnesium deficiency (medicine) Cyclic AMP GTP-Binding Protein alpha Subunits Gs medicine Animals Magnesium Magnesium ion Myocardium Body Weight Hemodynamics medicine.disease Diet Rats Endocrinology chemistry Potassium Calcium Magnesium Deficiency Adenylyl Cyclases |
| Zdroj: | Journal of Applied Physiology. 81:335-340 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/jappl.1996.81.1.335 |
| Popis: | Magnesium ions (Mg2+) play a crucial role in the activation and synthesis of guanine nucleotide-binding proteins (G proteins). However, there is no information about the influence of in vivo magnesium deficiency on the function and levels of G proteins. This study was done to investigate whether dietary magnesium deficiency alters function and levels of the two major myocardial G proteins, Gi alpha and Gs alpha. Severe hypomagnesemia and a significant reduction of myocardial magnesium occurred in rats fed a magnesium-deficient diet for 6 wk vs. rats fed a normal-magnesium diet (control). The magnesium-deficient rats developed focal myocardial lesions but their cardiac function was not impaired. Myocardial immunodetectable Gs alpha and Gi alpha levels of magnesium-deficient rats did not differ from control (Gs alpha: 2.39 +/- 0.52 vs. 2.76 +/- 0.72 arbitrary units/microgram protein, P > 0.05; Gi alpha: 1.60 +/- 0.52 vs. 1.89 +/- 0.30 arbitrary units/microgram protein, P > 0.05). Similarly, the function of Gs alpha and Gi alpha estimated by basal and ligand-stimulated adenylyl cyclase activity was not significantly different between the two groups of animals. The results show that dietary-derived magnesium deficiency sufficient to produce severe hypomagnesemia does not produce any significant change in levels or function of myocardial G proteins. |
| Databáze: | OpenAIRE |
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