The cytotoxic activity of the total alkaloids isolated from different parts of Solanum pseudocapsicum
| Autor: | Raghu Chandrashekhar Hariharpura, Prashanth Haranahalli Chandrappa Setty, Shrishailappa Badami, Preethi Vijayaraj, Suresh Bhojraj, Pottekkad Vijayan, Dhanaraj Sokkalingam Arumugam, Ashok Godavarthi |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Pharmaceutical Science Mice Solanum pseudocapsicum Alkaloids Botany Chlorocebus aethiops Cytotoxic T cell Animals Humans Cytotoxicity Cells Cultured Solanaceae Pharmacology Antitumor activity biology Plant Extracts Alkaloid General Medicine biology.organism_classification Antineoplastic Agents Phytogenic Biochemistry Cell culture Toxicity Vero cell Drug Screening Assays Antitumor Plant Structures |
| Zdroj: | Biologicalpharmaceutical bulletin. 27(4) |
| ISSN: | 0918-6158 |
| Popis: | The total alkaloid fractions of the methanolic extracts of the leaves, ripe fruits, roots, seeds and stem of Solanum pseudocapsicum were subjected to in-vitro cytotoxicity, short-term toxicity and long-term survival studies. All the five fractions exhibited potent activity. The total alkaloid fraction of leaves was found to be the most potent. The HT-29 cell line was the most sensitive to the fractions. The cytotoxic concentration (CTC(50)) values for all these fractions ranged between 0.39-0.91, 0.68-2.8, 0.92-3.56, 4.05-8.2, 3.28-5.65 and 0.95-5.55 microg/ml, respectively for HT-29, RD-228, A-549, HEp-2, B(16)F(10) and Vero cell lines. In short-term toxicity studies, the fractions showed 50% viability at 93-128 microg/ml for DLA cells and 141-189 microg/ml for human lymphocytes. In the long-term survival studies on the cell lines RD-228, HEp-2 and Vero, cells retained their regenerative capacities at concentrations below 8 microg/ml. The total alkaloids of the plant, especially from the leaves merit further investigations to identify the active constituents in animal models. |
| Databáze: | OpenAIRE |
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