Evaluation of PCR for cutaneous leishmaniasis diagnosis and species identification using filter paper samples in Panama, Central America
| Autor: | Aracelis Miranda, Hector Paz, José E. Calzada, Franklyn Samudio, G. Santamaría, Azael Saldaña, Kadir González |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Paper Pathology medicine.medical_specialty Panama Leishmaniasis Cutaneous Polymerase Chain Reaction Sensitivity and Specificity Specimen Handling law.invention Species Specificity Cutaneous leishmaniasis law Humans Medicine Species identification TE buffer Polymerase chain reaction Skin Filter paper business.industry Public Health Environmental and Occupational Health Leishmaniasis DNA General Medicine medicine.disease DNA extraction Infectious Diseases Female Parasitology business Filtration |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 106:544-548 |
| ISSN: | 0035-9203 |
| Popis: | Cutaneous leishmaniasis (CL) is a major vectorborne disease in Panama. In this study, the diagnostic performance and usefulness of two DNA extraction procedures from skin scraping samples collected on FTA filter paper for subsequent PCR diagnosis of CL was evaluated. A positive CL laboratory diagnosis was based on a positive parasitological test (Giemsa-stained smears or in vitro culture) and/or positive PCR test performed from skin scrapings collected in TE buffer (PCR-TE). Of 100 patients with skin lesions suggestive of CL, 82 (82%) were confirmed as CL positive. The sensitivity was calculated for each of the PCR approaches from samples collected on filter paper. The highest sensitivity was achieved by PCR-FTA processed by Chelex 100 (PCR-Chelex) (0.94). PCR-FTA extracted using the FTA purification reagent presented a lower sensitivity (0.60). Good concordance between routine PCR-TE and PCR-Chelex was observed (percent agreement=0.88, κ index=0.65). In conclusion, use of FTA filter paper for skin scraping collection combined with PCR is a reliable and convenient method for CL diagnosis in Panama, with comparable performance to the routine PCR method and with improved sensitivity compared with those of conventional parasitological methods. |
| Databáze: | OpenAIRE |
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