High-performance liquid chromatographic method for the quantitation of flecainide, a new antiarrhythmic, in human plasma and urine
| Autor: | Chang Sf, T.M. Welscher, A.M. Miller, R.E. Ober |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Flecainide
Chromatography Chromatography Gas Extraction (chemistry) General Chemistry Urine Standard curve Hexane chemistry.chemical_compound Column chromatography Pharmacokinetics chemistry Piperidines medicine Humans Gas chromatography Anti-Arrhythmia Agents Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of chromatography. 272(2) |
| Popis: | A simple, selective, sensitive, and accurate high-performance liquid chromatographic method for the quantitation of flecainide in human plasma and urine is described. The method is based on initially washing the sample with hexane followed by a single extraction with hexane. The extracted drug and internal standard are chromatographed on a Zorbax TMS column with a mobile phase consisting of acetonitrile-1% acetic acid in 0.01 M pentanesulfonate (45:55, v/v). The eluent is monitored at 308 nm. This method can routinely quantitate plasma or urine flecainide concentrations as low as 22 ng/ml with a 1-ml sample and 11 ng/ml with a 2-ml sample with no interference from endogenous substances and many drugs and their metabolites. The standard curve is linear over a concentration range of 22-1746 ng/ml. The precision and accuracy of the described method are suitable for monitoring flecainide levels in therapeutic, tolerance, and pharmacokinetic studies in humans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|