Role of Endothelium-Related Mechanisms in the Pathophysiology of Renal Ischemia/Reperfusion in Normal Rabbits
| Autor: | C. Caramelo, G. Espinosa, F. Manzarbeitia, M.R. Cernadas, G. Pe´rez Tejerizo, D. Tan, J.R. Mosquera, E. Digiuni, M. Monto´n, I. Milla´s, L. Hernando, S. Casado, A. Lo´pez-Farre´ |
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| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Pathology Time Factors Neutrophils Physiology Ischemia Renal function Arginine Kidney Nitric Oxide Renal Circulation Superoxide dismutase Reference Values Internal medicine medicine Animals Nitrites Peroxidase Renal circulation biology Renal ischemia business.industry Hemodynamics Kidney metabolism medicine.disease Vasodilation medicine.anatomical_structure Endocrinology Reperfusion biology.protein Endothelium Vascular Rabbits Cardiology and Cardiovascular Medicine business Kidney disease |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1524-4571 0009-7330 |
| Popis: | The present study addressed the effect of interventions aimed to increase NO in the setting of acute renal ischemia/reperfusion (I/R) in uninephrectomized rabbits. In the 60-minute post-I/R period, l -arginine+superoxide (O 2 •− ) dismutase (SOD) synergistically improved the renal functional (69.4% versus 10.4% of the pre-I/R glomerular filtration rate with or without l -arginine+SOD, respectively; P l -arginine+SOD, P l -arginine+SOD was no longer present at 24 and 48 hours (plasma creatinine in vehicle-treated versus l -arginine+SOD–treated animals [mg/100 mL]: 24 hours after I/R, 9.4±1.9 versus 8.07±0.65; 48 hours after I/R, 11.6±3.6 versus 9.7±0.9; P =NS in all the cases). Additional experiments were conducted using a milder 30-minute ischemic model, which showed no significant functional or histological protection by using l -arginine+SOD. In conclusion, our experiments disclosed the following: (1) the critical importance of the interaction between NO and O 2 •− in the acute protective effect of l -arginine (this effect not only improved renal function and histology but also reduced neutrophil accumulation) and (2) the discordance existing between the immediate protection afforded by l -arginine+SOD and the lack of protection observed at 24 and 48 hours. This finding suggests that a punctual intervention on the NO system at the time of I/R is not sufficient to reduce renal damage over the long term. |
| Databáze: | OpenAIRE |
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