Maternal inheritance of deltamethrin resistance in the salmon louse Lepeophtheirus salmonis (Krøyer) is associated with unique mtDNA haplotypes
| Autor: | Sally Boyd, Ross D. Houston, Greta Carmona-Antoñanzas, Joseph L. Humble, William Roy, David I. Bassett, James E. Bron, Michaël Bekaert, Karim Gharbi, Armin Sturm |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Non-Mendelian inheritance Insecticides Heredity Marine and Aquatic Sciences Disease Vectors Biochemistry Benzoates Insecticide Resistance chemistry.chemical_compound Salmon louse Pyrethrins Medicine and Health Sciences Energy-Producing Organelles Genetics Multidisciplinary Pyrethroid Mitochondrial DNA 3. Good health Mitochondria Insects Nucleic acids Genetic Mapping Chemistry Infectious Diseases Physical Sciences Medicine Maternal Inheritance Cellular Structures and Organelles Lice Research Article Arthropoda Forms of DNA Science Zoology Biology Bioenergetics DNA Mitochondrial Copepoda 03 medical and health sciences Sea Water Nitriles parasitic diseases Journal Article Parasitic Diseases Animals Haplotype Sire Ecology and Environmental Sciences Organisms Chemical Compounds Biology and Life Sciences Aquatic Environments Cell Biology DNA biology.organism_classification Invertebrates Marine Environments Insect Vectors Species Interactions 030104 developmental biology Deltamethrin chemistry Haplotypes Lepeophtheirus Uniparental Inheritance Earth Sciences Salts |
| Zdroj: | PLoS ONE, Vol 12, Iss 7, p e0180625 (2017) PLoS ONE Carmona-Antoñanzas, G, Bekaert, M, Humble, J L, Boyd, S, Roy, W, Bassett, D I, Houston, R D, Gharbi, K, Bron, J E & Sturm, A 2017, ' Maternal inheritance of deltamethrin resistance in the salmon louse Lepeophtheirus salmonis (Krøyer) is associated with unique mtDNA haplotypes ', PLoS ONE, vol. 12, no. 7, pp. e0180625 . https://doi.org/10.1371/journal.pone.0180625 ResearcherID |
| ISSN: | 1932-6203 |
| Popis: | Parasitic infections by the salmon louse, Lepeophtheirus salmonis (Krøyer), cause huge economic damage in salmon farming in the northern hemisphere, with combined treatment costs and production losses in 2014 having been estimated at US$ 350 million for Norway (annual production 1.25 million tonnes). The control of L. salmonis relies significantly on medicinal treatments, supplemented by non-pharmacological approaches. However, efficacy losses have been reported for several delousing agents, including the pyrethroid deltamethrin. The aim of the present study was to analyse the genetic basis of deltamethrin resistance in L. salmonis. Deltamethrin median effective concentrations (EC50) were 0.28 μg L-1 in the drug susceptible L. salmonis strain IoA-00 and 40.1 μg L-1 in the pyrethroid resistant strain IoA-02. IoA-00 and IoA-02 were crossed to produce families spanning one parental and three filial generations (P0, F1-F3). In three families derived from P0 crosses between an IoA-00 sire and an IoA-02 dam, 98.8% of F2 parasites (n = 173) were resistant, i.e. remained unaffected after exposure to 2.0 μg L-1 deltamethrin. F3 parasites from these crosses showed a deltamethrin EC50 of 9.66 μg L-1. In two families of the inverse orientation at P0 (IoA-02 sire x IoA-00 dam), 16.7% of F2 parasites were resistant (n = 84), while the deltamethrin EC50 in F3 animals was 0.26 μg L-1. The results revealed a predominantly maternal inheritance of deltamethrin resistance. The 15,947-nt mitochondrial genome was sequenced and compared among six unrelated L. salmonis strains and parasites sampled from wild salmon in 2010. IoA-02 and three further deltamethrin resistant strains, established from isolates originating from different regions of Scotland, showed almost identical mitochondrial haplotypes. In contrast, the mitochondrial genome was variable among susceptible strains and L. salmonis from wild hosts. Deltamethrin caused toxicity and depletion of whole body ATP levels in IoA-00 but not IoA-02 parasites. The maternal inheritance of deltamethrin resistance and its association with mitochondrial haplotypes suggests that pyrethroid toxicity in L. salmonis may involve molecular targets encoded by mitochondrial genes. |
| Databáze: | OpenAIRE |
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