Nanocomplexes of recombinant proteins and polysialic acid: Preparation, characteristics, and biological activity
| Autor: | M. G. Akimov, E. D. Shibanova, D. I. Bairamashvili, G. N. Zinchenko, M. Yu. Bobrov, Fomina-Ageeva Ev, Vladimir V. Bezuglov, Dmitry V. Klinov, Anatoly I. Miroshnikov, Natalia Gretskaya |
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| Rok vydání: | 2009 |
| Předmět: |
Glycopolymer
medicine.medical_treatment Antineoplastic Agents Interferon alpha-2 Biology Microscopy Atomic Force Biochemistry law.invention Mice chemistry.chemical_compound law In vivo Cell Line Tumor Granulocyte Colony-Stimulating Factor medicine Animals Humans Hypoglycemic Agents Insulin Bioorganic chemistry Cell Proliferation Cell growth Polysialic acid Organic Chemistry Interferon-alpha Biological activity Interferon-beta Recombinant Proteins Nanostructures chemistry Sialic Acids Recombinant DNA Electrophoresis Polyacrylamide Gel Rabbits Interferon beta-1b |
| Zdroj: | Russian Journal of Bioorganic Chemistry. 35:320-325 |
| ISSN: | 1608-330X 1068-1620 |
| DOI: | 10.1134/s1068162009030066 |
| Popis: | A preparation of nanocomplexes containing recombinant proteins (interferons alpha2b and beta1b, insulin, and human granulocyte colony stimulating factor) and natural polysialic acid (PSA) has been described. The incorporation of protein into the complex changes its electrophoretic mobility. Atomic force microscopy reveals the average size of 23-kD insulin complexes with PSA of 10-20 nm and demonstrates that more than 60% of glycopolymer molecules carry a single protein molecule. Experiments with cultured cells show that cytokines bound to polysialic acid retain their ability to regulate cell proliferation. Insulin bound to PSA has a prolonged hypoglycemic effect in vivo. |
| Databáze: | OpenAIRE |
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