Cytotoxic Deoxypodophyllotoxin Can Be Extracted in High Purity from Anthriscus sylvestris Roots by Supercritical Carbon Dioxide
| Autor: | Wim J. Quax, Christel L. C. Seegers, Rita Setroikromo, Pieter G. Tepper |
|---|---|
| Přispěvatelé: | Chemical and Pharmaceutical Biology, Nanotechnology and Biophysics in Medicine (NANOBIOMED), Biopharmaceuticals, Discovery, Design and Delivery (BDDD) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Anthriscus sylvestris Bioconversion Pharmaceutical Science 01 natural sciences Plant Roots etoposide Analytical Chemistry HeLa CELL-CYCLE ARREST Drug Discovery BIOCONVERSION Solvent free Supercritical carbon dioxide PODOPHYLLOTOXIN biology Chemistry Chromatography Supercritical Fluid APOPTOSIS Podophyllotoxin Molecular Medicine medicine.drug FLUID EXTRACTION CULTURES Antineoplastic Agents supercritical carbon dioxide extraction 03 medical and health sciences medicine Humans EPIPODOPHYLLOTOXIN PLANTS deoxypodophyllotoxin Pharmacology Chromatography 010405 organic chemistry Organic Chemistry Extraction (chemistry) Cell Cycle Checkpoints Carbon Dioxide biology.organism_classification Supercritical fluid 0104 chemical sciences 030104 developmental biology Complementary and alternative medicine LIGNANS A549 Cells Drugs Chinese Herbal HeLa Cells Apiaceae |
| Zdroj: | Planta Medica, 84(8), 544-550. GEORG THIEME VERLAG KG |
| ISSN: | 0032-0943 |
| Popis: | Deoxypodophyllotoxin is present in the roots of Anthriscus sylvestris. This compound is cytotoxic on its own, but it can also be converted into podophyllotoxin, which is in high demand as a precursor for the important anticancer drugs etoposide and teniposide. In this study, deoxypodophyllotoxin is extracted from A. sylvestris roots by supercritical carbon dioxide extraction. The process is simple and scalable. The supercritical carbon dioxide method extracts 75 – 80% of the total deoxypodophyllotoxin content, which is comparable to a single extraction by traditional Soxhlet. However, less polar components are extracted. The activity of the supercritical carbon dioxide extract containing deoxypodophyllotoxin was assessed by demonstrating that the extract arrests A549 and HeLa cells in the G2/M phase of the cell cycle. We conclude that biologically active deoxypodophyllotoxin can be extracted from A. sylvestris by supercritical carbon dioxide extraction. The method is solvent free and more sustainable compared to traditional methods. |
| Databáze: | OpenAIRE |
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