Addition of Polybrene improves stability of organophosphate hydrolase immobilized in poly(vinyl alcohol) cryogel carrier
| Autor: | Fatima M. Plieva, Sergei D. Varfolomeyev, Grigori M. Kazankov, Alexander K. Gladilin, Viktoriya S. Sergeeva, Elena Efremenko, T.A. Makhlis, Vladimir I. Lozinsky |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Vinyl alcohol Chromatography Ethanol Time Factors Paraoxon Dose-Response Relationship Drug Biophysics Temperature Biochemistry Phosphoric Monoester Hydrolases chemistry.chemical_compound Hydrolysis Enzyme Aryldialkylphosphatase chemistry Biocatalysis Covalent bond Pseudomonas medicine Escherichia coli Gels medicine.drug Hexadimethrine Bromide |
| Zdroj: | Journal of biochemical and biophysical methods. 51(2) |
| ISSN: | 0165-022X |
| Popis: | Organophosphate hydrolase, covalently attached to the beads of poly(vinyl alcohol) cryogel in the presence of Polybrene, was fivefold more stable in 15% (v/v) ethanol solution than the free enzyme. Immobilized biocatalyst, prepared with an addition of Polybrene, retained a half of its initial activity in 50% (v/v) aqueous ethanol solution, 90% of activity during 10 working cycles of Paraoxon hydrolysis and 85% of activity after storage in the 50 mM CHES buffer (pH 9.0) at room temperature for 2 months. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect