Proteomics Approach for the Discovery of Rheumatoid Arthritis Biomarkers Using Mass Spectrometry

Autor: Hee-Gyoo Kang, Hyo-Jin Kim, Yoo-Jin Lee, Arum Park, Hyunsong Son, Mi-Kyoung Lim, Sora Mun, Miji Shin, Jiyeong Lee
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Oncology
rheumatoid arthritis
Proteome
Vitamin D-binding protein
diagnosis
Proteomics
Mass Spectrometry
Arthritis
Rheumatoid
lcsh:Chemistry
0302 clinical medicine
skin and connective tissue diseases
lcsh:QH301-705.5
Spectroscopy
Principal Component Analysis
General Medicine
Middle Aged
Computer Science Applications
Rheumatoid arthritis
Biomarker (medicine)
biomarker
Female
musculoskeletal diseases
medicine.medical_specialty
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
proteomics
Internal medicine
medicine
Humans
Physical and Theoretical Chemistry
Molecular Biology
Aged
030203 arthritis & rheumatology
Autoimmune disease
business.industry
Organic Chemistry
Computational Biology
Guideline
medicine.disease
Rheumatology
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
business
Biomarkers
Rheumatism
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 18
International Journal of Molecular Sciences, Vol 20, Iss 18, p 4368 (2019)
ISSN: 1422-0067
DOI: 10.3390/ijms20184368
Popis: Rheumatoid arthritis is an autoimmune disease that causes serious functional loss in patients. Early and accurate diagnosis of rheumatoid arthritis may attenuate its severity. Despite a diagnosis guideline in the 2010 American College of Rheumatology (ACR)/European League Against Rheumatism (EULAR) classification criteria for rheumatoid arthritis, the practical difficulties in its diagnosis highlight the need of developing new methods for diagnosing rheumatoid arthritis. The current study aimed to identify rheumatoid arthritis diagnostic biomarkers by using a proteomics approach. Serum protein profiling was conducted using mass spectrometry, and five distinguishable biomarkers were identified therefrom. In the validation study, the five biomarkers were quantitatively verified by multiple reaction monitoring (MRM) analysis. Two proteins, namely serum amyloid A4 and vitamin D binding protein, showed high performance in distinguishing patients with rheumatoid arthritis from healthy controls. Logistic analysis was conducted to evaluate how accurately the two biomarkers distinguish patients with rheumatoid arthritis from healthy controls. The classification accuracy was 86.0% and 81.4% in patients with rheumatoid arthritis and in healthy controls, respectively. Serum amyloid A4 and vitamin D binding protein could be potential biomarkers related to the inflammatory response and joint destruction that accompany rheumatoid arthritis.
Databáze: OpenAIRE
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