Rice transcription factor GAMYB modulates bHLH142 and is homeostatically regulated by TDR during anther tapetal and pollen development
| Autor: | Hung-Chien Hsing, Swee-Suak Ko, Min-Jeng Li, Maurice Sun-Ben Ku, Tien-Kuan Chen, Wen-Hsiung Li, Yi Cheng Ho, Chun-Ping Yu, Yi-Jyun Lin, Ting-Ting Yang, Chung-Min Jhong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Stamen Plant Science Flowers Biology medicine.disease_cause 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Pollen medicine MYB Transcription factor Plant Proteins Tapetum Oryza sativa food and beverages Oryza Cell biology 030104 developmental biology Gibberellin Function (biology) 010606 plant biology & botany Transcription Factors |
| Zdroj: | Journal of experimental botany. 72(13) |
| ISSN: | 1460-2431 |
| Popis: | GIBBERELLIN MYB GENE (GAMYB), UNDEVELOPED TAPETUM1 (UDT1), TDR INTERACTING PROTEIN2 (TIP2/bHLH142), TAPETUM DEGENERATION RETARDATION (TDR), and ETERNAL TAPETUM 1/DELAYED TAPETUM DEGENERATION (EAT1/DTD) are important transcription factors that play a crucial role during pollen development in rice. This study demonstrates that bHLH142 acts downstream of UDT1 and GAMYB and works as a ‘hub’ in these two pollen pathways. We show that GAMYB modulates bHLH142 expression through specific binding to the MYB motif of the bHLH142 promoter during the early stage of pollen development, while TDR acts as a transcriptional repressor of the GAMYB modulation of bHLH142 by binding to the E-box close to the MYB motif on the promoter. Altered expression of these transcription factors highlights that a tight, precise, and coordinated regulation among them is essential for normal pollen development. Most notably, we show that the regulatory pathways of GAMYB and UDT1 rely on bHLH142 in a direct and indirect manner, respectively, and function in different tissues with distinct biological roles during pollen development. This study advances our understanding of the molecular mechanisms of rice pollen development. |
| Databáze: | OpenAIRE |
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