Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology
| Autor: | Groenen, P.J.T.A., Brand, M. van den, Kroeze, L., Amir, A., Hebeda, K.M. |
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| Rok vydání: | 2023 |
| Předmět: |
Cancer Research
All institutes and research themes of the Radboud University Medical Center Oncology Urological cancers Radboud Institute for Health Sciences [Radboudumc 15] Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2] Rare cancers Radboud Institute for Molecular Life Sciences [Radboudumc 9] |
| Zdroj: | Frontiers in Oncology, 13 |
| ISSN: | 2234-943X |
| Popis: | Clonality assessment using the unique rearrangements of immunoglobulin (IG) and T-cell receptor (TR) genes in lymphocytes is a widely applied supplementary test for the diagnosis of B-cell and T-cell lymphoma. To enable a more sensitive detection and a more precise comparison of clones compared with conventional clonality analysis based on fragment analysis, the EuroClonality NGS Working Group developed and validated a next-generation sequencing (NGS)-based clonality assay for detection of the IG heavy and kappa light chain and TR gene rearrangements for formalin-fixed and paraffin-embedded tissues. We outline the features and advantages of NGS-based clonality detection and discuss potential applications for NGS-based clonality testing in pathology, including site specific lymphoproliferations, immunodeficiency and autoimmune disease and primary and relapsed lymphomas. Also, we briefly discuss the role of T-cell repertoire of reactive lymphocytic infiltrations in solid tumors and B-lymphoma. |
| Databáze: | OpenAIRE |
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