Norovirus genotypes implicated in two oyster-related illness outbreaks in Ireland
| Autor: | Sinéad Keaveney, Paulina Rajko-Nenow, John Flannery, A. McINTYRE, William Doré |
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| Rok vydání: | 2013 |
| Předmět: |
Oyster
Veterinary medicine Genotype Epidemiology Biology Real-Time Polymerase Chain Reaction medicine.disease_cause Disease Outbreaks Feces fluids and secretions biology.animal medicine Animals Humans Caliciviridae Infections Molecular Epidemiology Molecular epidemiology Phylogenetic tree Reverse Transcriptase Polymerase Chain Reaction Norovirus food and beverages Outbreak Viral Load Original Papers Ostreidae Virology Gastroenteritis Reverse transcription polymerase chain reaction Infectious Diseases RNA Viral Cloning procedures Ireland |
| Zdroj: | Epidemiol Infect |
| ISSN: | 1469-4409 0950-2688 |
| DOI: | 10.1017/s0950268813003014 |
| Popis: | SUMMARYWe investigated norovirus (NoV) concentrations and genotypes in oyster and faecal samples associated with two separate oyster-related outbreaks of gastroenteritis in Ireland. Quantitative analysis was performed using real-time quantitative reverse transcription polymerase chain reaction and phylogenetic analysis was conducted to establish the NoV genotypes present. For both outbreaks, the NoV concentration in oysters was >1000 genome copies/g digestive tissue and multiple genotypes were identified. In faecal samples, GII.13 was the only genotype detected for outbreak 1, whereas multiple genotypes were detected in outbreak 2 following the application of cloning procedures. While various genotypes were identified in oyster samples, not all were successful in causing infection in consumers. In outbreak 2 NoV GII.1 was identified in all four faecal samples analysed and NoV GII concentrations in faecal samples were >108copies/g. This study demonstrates that a range of NoV genotypes can be present in highly contaminated oysters responsible for gastroenteritis outbreaks. |
| Databáze: | OpenAIRE |
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