Evaluation of the influence of protein precipitation prior to on-line SPE-LC-API/MS procedures using multivariate data analysis
| Autor: | Maurice H. J. Selman, Ivano Marchi, Serge Rudaz, Jean-Luc Veuthey |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Pyrrolidines
Electrospray ionization Clinical Biochemistry Analytical chemistry Photoionization Flunitrazepam Mass spectrometry Biochemistry Online Systems Mass Spectrometry Analytical Chemistry Liquid chromatography–mass spectrometry Ionization Fluoxetine Protein precipitation Chemical Precipitation Solid phase extraction Solid Phase Extraction/methods Mass Spectrometry/methods Cholecalciferol ddc:615 Chromatography Alanine Atmospheric pressure Chemistry Blood Proteins/isolation & purification Solid Phase Extraction Chromatography Liquid/methods Cell Biology General Medicine Alanine/analogs & derivatives Blood Proteins Fluoxetine/analogs & derivatives Multivariate Analysis Methadone Chromatography Liquid |
| Zdroj: | Journal of Chromatography. B, Vol. 845, No 2 (2007) pp. 244-52 |
| ISSN: | 1570-0232 |
| Popis: | Matrix effects on mass spectrometry (MS) response were investigated with three atmospheric pressure ionization (API) sources after on-line solid-phase extraction (SPE) of human plasma. On-line SPE was evaluated with one restricted access material (RAM), two large particle supports (LPS) and one monolith. A sample protein precipitation (PP) with acetonitrile (2:1) and a direct injection were tested. Principal component analysis (PCA) was performed to simplify data presentation and interpretation. Protein precipitation was found to be mandatory for reducing signal modification. Regarding sensitivity towards matrix effects after PP, atmospheric pressure photoionization (APPI) was globally the least sensitive ionization mode while electrospray ionization ESI was the most sensitive. |
| Databáze: | OpenAIRE |
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