An important resource for understanding bio-adhesion mechanisms: Cement gland transcriptomes of two goose barnacles, Pollicipes pollicipes and Lepas anatifera (Cirripedia, Thoracica)

Autor: Vitor Vasconcelos, Isabel Cunha, L. Filipe C. Castro, Elena Sarropoulou, André M. Machado
Přispěvatelé: CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental
Rok vydání: 2019
Předmět:
Zdroj: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Marine Genomics
ISSN: 1874-7787
DOI: 10.1016/j.margen.2018.11.001
Popis: Barnacles are sessile organisms globally distributed in marine and estuarine environments. The production of protein-based adhesives by cement glands is crucial for these organisms and plays a vital role in substrate attachment, thus inspiring biotechnological applications. Moreover, barnacles belong to bio-fouling communities, whose fixation onto man-made surfaces is of interest in terms of control and imposes an enormous economic cost on maritime industries. Here, we generated the first de novo assembly of cement gland transcriptomes of Pollicipes pollicipes (rocky-shore goose barnacle) and Lepas anatifera (pelagic goose barnacle) (Cirripedia, Thoracica), to generate molecular resources for understanding the bio-adhesion process in Cirripedia and to provide data for biotechnology. Via the Illumina next-generation sequencing technology, a total of 43.7 and 40.2 million (M) paired-end (PE) clean reads were obtained for P. pollicipes (PP) and L. anatifera (LA), respectively. De novo assemblies resulted in 116,377 transcripts for PP and 94,143 for LA, with N50 s of 1181 and 1028 bp, respectively. The Benchmarking Universal Single-Copy Orthologs (BUSCO) showed 78.7% (PP) and 91.7% (LA) of transcriptome completeness, in terms of expected gene content, for the Arthropoda phylum in each species. These results represent a substantial resource for expanding the knowledge on Cirripedia adhesion mechanisms and for contributions to the gene annotation of Crustacea where molecular resources are presently insufficient. © 2018 Elsevier This work was supported by the CY-Sensors project (POCI-01-0145-FEDER-032359 ) through Fundação para a Ciência e a Tecnologia (FCT), Portugal and the INNOVMAR project: Innovation and Sustainability in the Management and Exploitation of Marine Resources ( NORTE-01-0145-FEDER-000035 ), Research Line NOVELMAR. The Transnational Access program of the European Marine Biology Resource Infrastructure Cluster (EMBRIC), European Union project (ID: 654008 ) funded under H2020 - EU.1.4.1.1 “Developing new world-class research infrastructures” provided support for transcriptome analysis at the HCMR Biocluster in Crete (Project No. 2757 ) and the mission costs of I·C. to Crete. I.C was supported by and FCT , Portugal fellowship ( SFRH/BPD/110020/2015 ). The research of A.M.M. and L.F.C.C. was supported by the MarInfo – Integrated Platform for Marine Data Acquisition and Analysis ( NORTE-01-0145-FEDER-000031 ), a project supported by the North Portugal Regional Operational Program ( NORTE 2020 ), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), European-Union. We would also like to acknowledge C. Frangoulis and E. Pettas from the Institute of Oceanography at HCMR for the sampling of L. anatifera in Gournes, Crete, and T. Neuparth for helping with RNA extraction. Appendix A
Databáze: OpenAIRE
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