The interaction of a Huntington disease factor with receptors for the neurotoxin kainic acid
| Autor: | A. Ian Smith, Murray Morrison, Wendy E. J. Watson, Graham A.R. Johnston, Peter R. Dodd, Anita Ruwoldt, Ronald S. Walls |
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| Rok vydání: | 1991 |
| Předmět: |
Leukocyte migration
Kainic acid Synaptic Membranes Biology In Vitro Techniques Inhibitory postsynaptic potential Biochemistry High-performance liquid chromatography Cellular and Molecular Neuroscience chemistry.chemical_compound Receptors Kainic Acid medicine Leukocytes Neurotoxin Animals Humans Receptor Chromatography High Pressure Liquid Cerebral Cortex Kainic Acid Brain Glutamic acid Human brain Corpus Striatum Rats Receptors Neurotransmitter medicine.anatomical_structure Huntington Disease chemistry Spectrophotometry Ultraviolet Neurology (clinical) Subcellular Fractions |
| Zdroj: | Metabolic brain disease. 6(4) |
| ISSN: | 0885-7490 |
| Popis: | A factor from mammalian and human brain, which inhibits the rate of migration of leukocytes obtained from sufferers from Huntington disease (Walls and Ruwoldt, 1984), inhibited the specific binding of the neurotoxin [3H]kainic acid to rat brain synaptic membranes. The factor was present in sucrose-particulate but not in soluble fractions from rat sub-cortical tissue, and was destroyed by tryptic digestion. Whereas an ammonium sulfate fraction of direct saline extracts of brain (Walls and Ruwoldt, 1984) gave poor chromatography on HPLC, prior separation of a sucrose-particulate fraction resulted in much improved chromatography. There was a good concordance between leukocyte migration inhibitory activity and [3H]kainic acid binding inhibitory activity. The factor may be an endogenous modulator of the kainic acid subset of receptors for the excitatory neurotransmitter glutamic acid. |
| Databáze: | OpenAIRE |
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