Distinct small non-coding RNA landscape in the axons and released extracellular vesicles of developing primary cortical neurons and the axoplasm of adult nerves
| Autor: | Raquel Mesquita-Ribeiro, Rafael Sebastián Fort, Alex Rathbone, Joaquina Farias, Cristiano Lucci, Victoria James, Jose Sotelo-Silveira, Maria Ana Duhagon, Federico Dajas-Bailador |
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| Přispěvatelé: | Mesquita-Ribeiro R, Fort Canobra Rafael S, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología., Rathbone Alex, Farías Joaquina, IIBCE, Lucci Cristiano, James Victoria, Sotelo Silveira José Roberto, IIBCE, Duhagon María Ana, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología., Dajas-Bailador F |
| Rok vydání: | 2021 |
| Předmět: |
sncRNAs
Biochemistry & Molecular Biology MICRORNAS Neuronal Outgrowth Cell Communication Cell Fractionation Axon Extracellular Vesicles 03 medical and health sciences 0302 clinical medicine RNA Transfer Humans tRNA-derived fragments Molecular Biology FRAGMENTS TARGETING MAP1B 030304 developmental biology EXOSOMES Neurons axon 0303 health sciences Science & Technology IDENTIFICATION PROFILING REVEALS Computational Biology High-Throughput Nucleotide Sequencing Biological Transport Molecular Sequence Annotation Cell Biology Extracellular vesicles Axons nervous system miRNAs LOCAL PROTEIN-SYNTHESIS GROWTH Nucleic Acid Conformation RNA Small Untranslated TRANSLATION MESSENGER-RNA Life Sciences & Biomedicine 030217 neurology & neurosurgery Research Article Research Paper Subcellular Fractions |
| Zdroj: | RNA Biology article-version (VoR) Version of Record COLIBRI Universidad de la República instacron:Universidad de la República |
| ISSN: | 1555-8584 1547-6286 |
| DOI: | 10.1080/15476286.2021.2000792 |
| Popis: | Neurons have highlighted the needs for decentralized gene expression and specific RNA function in somato-dendritic and axonal compartments, as well as in intercellular communication via extracellular vesicles (EVs). Despite advances in miRNA biology, the identity and regulatory capacity of other small non-coding RNAs (sncRNAs) in neuronal models and local subdomains has been largely unexplored.We identified a highly complex and differentially localized content of sncRNAs in axons and EVs during early neuronal development of cortical primary neurons and in adult axons invivo. This content goes far beyond miRNAs and includes most known sncRNAs and precisely processed fragments from tRNAs, sno/snRNAs, Y RNAs and vtRNAs. Although miRNAs are the major sncRNA biotype in whole-cell samples, their relative abundance is significantly decreased in axons and neuronal EVs, where specific tRNA fragments (tRFs and tRHs/tiRNAs) mainly derived from tRNAs Gly-GCC, Val-CAC and Val-AAC predominate. Notably, although 5'-tRHs compose the great majority of tRNA-derived fragments observed invitro, a shift to 3'-tRNAs is observed in mature axons invivo.The existence of these complex sncRNA populations that are specific to distinct neuronal subdomains and selectively incorporated into EVs, equip neurons with key molecular tools for spatiotemporal functional control and cell-to-cell communication. ispartof: RNA BIOLOGY vol:18 issue:sup2 pages:832-855 ispartof: location:United States status: published |
| Databáze: | OpenAIRE |
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