The use of silane-coated silica particles for density gradient centrifugation in in-vitro fertilization
| Autor: | Brita Söderlund, Kersti Lundin |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
medicine.medical_treatment Semen Cell Separation Fertilization in Vitro Biology Teratozoospermia Andrology Human fertilization Pregnancy Centrifugation Density Gradient medicine Humans reproductive and urinary physiology Sperm motility In vitro fertilisation urogenital system Rehabilitation Povidone Obstetrics and Gynecology Silanes Silicon Dioxide Spermatozoa Sperm Microspheres Pregnancy rate Treatment Outcome Reproductive Medicine Immunology Sperm Motility Female Percoll |
| Zdroj: | Human Reproduction. 15:857-860 |
| ISSN: | 1460-2350 |
| DOI: | 10.1093/humrep/15.4.857 |
| Popis: | Silane-coated silica particles (PureSperm) were evaluated as an alternative to Percoll for gradient separation of spermatozoa, for use in assisted reproduction. Recovery of motile and morphologically normal spermatozoa after using a four-layer Percoll and a two- and four-layer PureSperm gradient respectively was recorded. In-vitro fertilization (IVF) results after using PureSperm for the sperm preparation were also evaluated. No difference in sperm recovery or sperm motility was found when comparing the use of Percoll and the four-layer gradient of PureSperm. When using a two-layer PureSperm gradient, motility was significantly decreased (P < 0.05) compared to Percoll. Normal sperm morphology increased from 8-17.2% after using Percoll and to 12.7% and 11.4% after using a four-layer and a two-layer PureSperm gradient respectively. All gradient preparations showed a significant decrease in the teratozoospermia index compared to the ejaculate (P < 0.01). No significant differences in IVF results regarding fertilization and pregnancy rates were found when PureSperm or the swim-up technique were used for the sperm preparation. PureSperm seems to be an acceptable alternative to Percoll but although the percentage of sperm recovery was higher after PureSperm we still recommend the swim-up technique to be the first choice, as a higher percentage of progressive motile spermatozoa is obtained without using other chemicals than IVF culture medium. |
| Databáze: | OpenAIRE |
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