Characterization of Gordonia sp. strain F.5.25.8 capable of dibenzothiophene desulfurization and carbazole utilization
| Autor: | Alvaro C. Leitão, Carla P. S. Matta, Monica Y. M. Sassaki, Marcelo de Pádula, Orlando B. Martins, Celuta S. Alviano, Daniela S. Alviano, Juliana V. Bevilaqua, G. V. Sebastián, Claudia Maria Soares Ribeiro, S. C. C. Santos, Lucy Seldin |
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| Rok vydání: | 2006 |
| Předmět: |
Nitrogen
Carbazoles chemistry.chemical_element Thiophenes Polymerase Chain Reaction Applied Microbiology and Biotechnology chemistry.chemical_compound RNA Ribosomal 16S Gordonia Bacterium Strain (chemistry) biology Carbazole Genes rRNA Sequence Analysis DNA General Medicine 16S ribosomal RNA biology.organism_classification Sulfur Culture Media Biochemistry chemistry Dibenzothiophene Primer (molecular biology) Rhodococcus Biotechnology Dibenzothiophene desulfurization |
| Zdroj: | Applied Microbiology and Biotechnology. 71:355-362 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-005-0154-z |
| Popis: | A dibenzothiophene (DBT)-degrading bacterial strain able to utilize carbazole as the only source of nitrogen was identified as Gordonia sp. F.5.25.8 due to its 16S rRNA gene sequence and phenotypic characteristics. Gas chromatography (GC) and GC-mass spectroscopy analyses showed that strain F.5.25.8 transformed DBT into 2-hydroxybiphenyl (2-HBP). This strain was also able to grow using various organic sulfur or nitrogen compounds as the sole sulfur or nitrogen sources. Resting-cell studies indicated that desulfurization occurs either in cell-associated or in cell-free extracts of F.5.25.8. The biological responses of F.5.25.8 to a series of mutagens and environmental agents were also characterized. The results revealed that this strain is highly tolerant to DNA damage and also refractory to induced mutagenesis. Strain F.5.25.8 was also characterized genetically. Results showed that genes involved in desulfurization (dsz) are located in the chromosome, and PCR amplification was observed with primers dszA and dszB designed based on Rhodococcus genes. However, no amplification product was observed with the primer based on dszC. |
| Databáze: | OpenAIRE |
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