The lipid lowering and antioxidative stress potential of polysaccharide from Auricularia auricula prepared by enzymatic method
| Autor: | Yongjun Zhang, Jingjing Tan, Qiqi Huang, Jiangying Zeng, Ge Wang, Shuang Chen, Bin Xiao, jing yao, Tao Feng |
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| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
medicine.medical_treatment Down-Regulation Xylose Chemical Fractionation Polysaccharide Biochemistry Antioxidants Superoxide dismutase chemistry.chemical_compound Auricularia Picrates Structural Biology Glucosamine medicine Animals Food science Benzothiazoles Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Ethanol precipitation Triglycerides Adiposity Hypolipidemic Agents chemistry.chemical_classification ABTS biology Biphenyl Compounds Metalloendopeptidases Fungal Polysaccharides General Medicine Glucuronic acid chemistry biology.protein Sulfonic Acids |
| Zdroj: | International journal of biological macromolecules. 187 |
| ISSN: | 1879-0003 |
| Popis: | An efficient extraction method of Auricularia auricula polysaccharides (AAPs) by neutral protease was developed and optimized by response surface methodology. AAPs were graded by stepwise ethanol precipitation, the fraction with high recovery rate and strong radical scavenging rate were obtained, then its antioxidant and lipid lowering effect were studied using Caenorhabditis elegans as model organism. The extract yield and ABTS+ scavenging rates of AAPs could reach 14.90% and 86.0% at 50 °C, 75 mL/g of liquid-to-material ratio and pH 9.0. AAP3 obtained by 15% ethanol was a heteropolysaccharide comprised of mannose, glucose, glucuronic acid, xylose, galactose and glucosamine. AAP3 could significantly prolong the lifespan of C. elegans and enhance the activity of antioxidant enzymes including superoxide dismutase (SOD), catalases (CAT) at 0.25 mg/mL (p 1.6 fold) at 0.25 mg/mL. Besides, AAP3 could significantly reduce the level of body fat and triglyceride in C. elegans (p |
| Databáze: | OpenAIRE |
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