Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes

Autor: Julius Osaso, Yoshikazu Honda, Trushar Shah, James Gachanja, Evelyne Abuya, Elias Awino, Anthony M. Muthiani, Roger Pelle, Duncan Mwangi, J.C. Audonnet, Pierre van der Bruggen, Rosemary Saya, John K. Nyanjui, Claude Wildmann, David Ndegwa, Ferdinand Mbwika, Subhash Morzaria, Malcolm J. Gardner, Evans L. N. Taracha, Sheena M. Loosmore, Mat Yamage, Nyerhovwo J. Tonukari, Simon P. Graham, Claire M. Fraser, Anthony E. Luyai, Etienne P. de Villiers, Sarah C. Gilbert, Ian Maudlin, Fredrick O. Onono, Moses N. Njahira, Richard P. Bishop, E. Jane Glew, Vishvanath Nene
Přispěvatelé: UCL - Autre
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Zdroj: Proceedings of the National academy of sciences of the United States of America, Vol. 103, no. 9, p. 3286-3291 (2006)
ISSN: 1091-6490
0027-8424
Popis: East Coast fever, caused by the tick-borne intracellular apicomplexan parasiteTheileria parva, is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte transformation is a unique cancer-like condition that is reversible with parasite removal. Schizont-infected cell-directed CD8+cytotoxic T lymphocytes (CTL) constitute the dominant protective bovine immune response after a single exposure to infection. However, the schizont antigens targeted byT. parva-specific CTL are undefined. Here we show the identification of five candidate vaccine antigens that are the targets of MHC class I-restricted CD8+CTL from immune cattle. CD8+T cell responses to these antigens were boosted inT. parva-immune cattle resolving a challenge infection and, when used to immunize naïve cattle, induced CTL responses that significantly correlated with survival from a lethal parasite challenge. These data provide a basis for developing a CTL-targeted anti-East Coast fever subunit vaccine. In addition, orthologs of these antigens may be vaccine targets for other apicomplexan parasites.
Databáze: OpenAIRE
načítá se...