Effects of dexamethasone on insulin receptor in aging
| Autor: | Mojca Stojiljkovic, Snezana Radivojsa, Zorica Zakula, Goran Koricanac, Esma R. Isenovic, Nevena Ribarac-Stepic |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Blood Glucose
Male Aging medicine.medical_specialty medicine.medical_treatment dexamethasone liver Dexamethasone General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Internal medicine polycyclic compounds medicine Animals Insulin RNA Messenger insulin receptor Binding site Glucocorticoids 030304 developmental biology General Environmental Science 0303 health sciences biology Chemistry Erythrocyte Membrane aging Rats Inbred Strains Plasma levels Receptor Insulin Rats 3. Good health Dissociation constant Insulin receptor Endocrinology Liver Neurology Mrna level Rat liver erythrocytes biology.protein hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Acta Biologica Hungarica |
| ISSN: | 1588-256X 0236-5383 |
| DOI: | 10.1556/abiol.59.2008.1.2 |
| Popis: | The aim of this study was to examine the effects of dexamethasone (Dex) on functional properties of the rat insulin receptor (IR). Male Mill Hill hooded rats, 3, 6, 12, 18 and 21 months old, were injected with Dex (4 mg/kg) and rat liver and erythrocytes were used for experiments 18 h after Dex administration. Treatment with Dex lowered the specific binding (SB) of insulin ( INS) in the liver of 3- and 18-month-old rats and concentration of INS binding sites (N-1, N-2) and the dissociation constant of low-affinity binding sites (Kd(2)) in the liver of 6- and 18-month-old rats. In addition, Dex treatment lowered the liver IR protein level in all analyzed groups, except 21-month-old rats where it remained unchanged, but raised the IR mRNA level in 18-month-old rats. In erythrocytes, treatment with Dex decreased SB and Kd2 ( in animals 3 and 6 months old) and N1 ( in ones 3 and 18 months old). Following Dex treatment, the INS plasma level increased ( in rats 3, 18 and 21 months old), while glucose (Glu) concentration increased in 3 and 12 months old, but decreased in 6- and 21-month-old rats. In summary, Dex exerts the strongest effect on the erythrocyte IR of 3- and 6- month-old rats and the hepatic IR of 18-month-old rats. IR in both tissues is almost insensitive to Dex in 12- and 21-month-old rats. The pattern of age-related changes of IR induced by Dex does not correlate with changes of plasma Glu and INS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink