Continuous Electrical Stimulation Affects Initial Growth and Proliferation of Adipose-Derived Stem Cells
| Autor: | Rainer Bader, Vivien Engel, Robert David, Bilal Al-Nawas, Anika Jonitz-Heincke, Bernhard Frerich, Daniel G. E. Thiem, Michael Dau, Peer W. Kämmerer, Dirk Timmermann, Franz Plocksties, Nadja Engel, Anna Skorska |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
proliferation Medicine (miscellaneous) Adipose tissue Stimulation General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences 0302 clinical medicine Tissue engineering electrical stimulation lcsh:QH301-705.5 continuous stimulation Chemistry Cell growth 030206 dentistry In vitro Cell biology Cell cycle analysis 030104 developmental biology lcsh:Biology (General) Apoptosis tissue engineering human adipose-derived stem cells Stem cell alternating current |
| Zdroj: | Biomedicines Biomedicines, Vol 8, Iss 482, p 482 (2020) Volume 8 Issue 11 |
| ISSN: | 2227-9059 |
| Popis: | The aim of the study was to establish electrical stimulation parameters in order to improve cell growth and viability of human adipose-derived stem cells (hADSC) when compared to non-stimulated cells in vitro. hADSC were exposed to continuous electrical stimulation with 1.7 V AC/20 Hz. After 24, 72 h and 7 days, cell number, cellular surface coverage and cell proliferation were assessed. In addition, cell cycle analysis was carried out after 3 and 7 days. After 24 h, no significant alterations were observed for stimulated cells. At day 3, stimulated cells showed a 4.5-fold increase in cell numbers, a 2.7-fold increase in cellular surface coverage and a significantly increased proliferation. Via cell cycle analysis, a significant increase in the G2/M phase was monitored for stimulated cells. Contrastingly, after 7 days, the non-stimulated group exhibited a 11-fold increase in cell numbers and a 4-fold increase in cellular surface coverage as well as a significant increase in cell proliferation. Moreover, the stimulated cells displayed a shift to the G1 and sub-G1 phase, indicating for metabolic arrest and apoptosis initiation. In accordance, continuous electrical stimulation of hADSC led to a significantly increased cell growth and proliferation after 3 days. However, longer stimulation periods such as 7 days caused an opposite result indicating initiation of apoptosis. |
| Databáze: | OpenAIRE |
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