An ultrasensitive and rapid 'sample-to-answer' microsystem for on-site monitoring of SARS-CoV-2 in aerosols using 'in situ' tetra-primer recombinase polymerase amplification

Autor: Shanglin, Li, Bao, Li, Xinyue, Li, Ce, Liu, Xiao, Qi, Yin, Gu, Baobao, Lin, Lingli, Sun, Lan, Chen, Bingqian, Han, Jiazhen, Guo, Yanyi, Huang, Shuangsheng, Wu, Lili, Ren, Jianbin, Wang, Jingwei, Bai, Jianxin, Ma, Maosheng, Yao, Peng, Liu
Rok vydání: 2023
Předmět:
Zdroj: Biosensors and Bioelectronics. 219:114816
ISSN: 0956-5663
DOI: 10.1016/j.bios.2022.114816
Popis: Airborne transmissibility of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted the urgent need for aerosol monitoring of SARS-CoV-2 to prevent sporadic outbreaks of COVID-19. The inadequate sensitivity of conventional methods and the lack of an on-site detection system limited the practical SARS-CoV-2 monitoring of aerosols in public spaces. We have developed a novel SARS-CoV-2-in-aerosol monitoring system (SIAMs) which consists of multiple portable cyclone samplers for collecting aerosols from several venues and a sensitive "sample-to-answer" microsystem employing an integrated cartridge for the analysis of SARS-CoV-2 in aerosols (iCASA) near the sampling site. By seamlessly combining viral RNA extraction based on a chitosan-modified quartz filter and "in situ" tetra-primer recombinase polymerase amplification (tpRPA) into an integrated microfluidic cartridge, iCASA can provide an ultra-high sensitivity of 20 copies/mL, which is nearly one order of magnitude greater than that of the commercial kit, and a short turnaround time of 25 min. By testing various clinical samples of nasopharyngeal swabs, saliva, and exhaled breath condensates obtained from 23 COVID-19 patients, we demonstrate that the positive rate of our system was 3.3 times higher than those of the conventional method. Combining with multiple portable cyclone samplers, we detected 52.2% (12/23) of the aerosol samples, six times higher than that of the commercial kit, collected from the isolation wards of COVID-19 patients, demonstrating the excellent performance of our system for SARS-CoV-2-in-aerosol monitoring. We envision the broad application of our microsystem in aerosol monitoring for fighting the COVID-19 pandemic.
Databáze: OpenAIRE
Externí odkaz: