Spectrophotometry of Hemoglobin: Absorption Spectra of Bovine Oxyhemoglobin, Deoxyhemoglobin, Carboxyhemoglobin, and Methemoglobin
| Autor: | W G Zijlstra, A Buursma |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
absorption spectra
Absorption spectroscopy OSM3 PH Physiology Analytical chemistry Biochemistry Methemoglobin hemoglobinometry chemistry.chemical_compound Spectrophotometry carboxyhemoglobin medicine cyanmethemoglobin Solubility methemoglobin Molecular Biology Chromatography medicine.diagnostic_test bos tauris DERIVATIVES hemoglobin OXYGEN-SATURATION chemistry Carboxyhemoglobin hemiglobin Hemoglobin Absorption (chemistry) Hemoglobinometry |
| Zdroj: | Comparative biochemistry and physiology b-Biochemistry & molecular biology, 118(4), 743-749. ELSEVIER SCIENCE INC |
| ISSN: | 1096-4959 0305-0491 |
| DOI: | 10.1016/s0305-0491(97)00230-7 |
| Popis: | The absorptivity at 540 nm of bovine hemiglobincyanide (cyanmethemoglobin) was determined on the basis of the iron content and found to be equal to the established value for human hemiglobincyanide (11.0 L . mmol(-1).cm(-1)). On this basis the absorption spectra of the common derivatives were determined for bovine hemoglobin. There proved to be only slight differences in the oxyhemoglobin, deoxyhemoglobin, and carboxyhemoglobin spectra between bovine and human hemoglobin. For comparison of the methemoglobin spectra a new series of measurements was made for human hemoglobin. As also found in the rat, the methemoglobin spectrum of bovine blood differed considerably from that in the human. These differences should be taken into account in multicomponent analysis. (C) 1991 Elsevier Science Inc. |
| Databáze: | OpenAIRE |
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