Suitability of PER.C6 (R) cells to generate epidemic and pandemic influenza vaccine strains by reverse genetics

Autor: Jaap Goudsmit, Ronald Vogels, L. Hartgroves, C. Ophorst, Lisette A. H. M. Cornelissen, Isabelle Legastelois, Menzo J. E. Havenga, Wendy S. Barclay, Wouter Koudstaal, David Zuijdgeest, O. S. de Leeuw, Jerome Custers, Martijn Sieuwerts, E.A. de Boer-Luijtze
Přispěvatelé: TNO Kwaliteit van Leven, Other departments
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Biomedical Research
Influenza vaccine
Cell Culture Techniques
Viral Plaque Assay
Biology
dna
system
medicine.disease_cause
Antibodies
Viral
Transfection
Severity of Illness Index
Microbiology
Cell Line
Mice
Influenza A Virus
H1N1 Subtype
CVI - Divisie Virologie
establishment
Reassortant Viruses
Influenza
Human
medicine
Influenza A virus
Animals
Humans
rna-polymerase
Virus quantification
Plaque Assay
Mice
Inbred BALB C
General Veterinary
General Immunology and Microbiology
Influenza A Virus
H5N1 Subtype
Influenza A Virus
H3N2 Subtype
Public Health
Environmental and Occupational Health
Hemagglutination Inhibition Tests
Virology
Survival Analysis
Influenza A virus subtype H5N1
Reverse genetics
Vaccination
Infectious Diseases
Influenza Vaccines
Inactivated vaccine
Molecular Medicine
rescue
Female
a virus generation
CVI - Division Virology
Zdroj: Vaccine 27 (2009) 19
Vaccine, 27(19), 2588-2593
Vaccine, 27(19), 2588-2593. Elsevier BV
Vaccine, 19, 27, 2588-2593
ISSN: 0264-410X
Popis: Reverse genetics, the generation of influenza viruses from cDNA, presents a rapid method for creating vaccine strains. The technique necessitates the use of cultured cells. Due to technical and regulatory requirements, the choice of cell lines for production of human influenza vaccines is limited. PER.C6® cells, among the most extensively characterized and documented cells, support growth of all influenza viruses tested to date, and can be grown to high densities in large bioreactors in the absence of serum or micro carriers. Here, the suitability of these cells for the generation of influenza viruses by reverse genetics was investigated. A range of viruses reflective of vaccine strains was rescued exclusively using PER.C6 cells by various transfection methods, including an animal component-free procedure. Furthermore, a whole inactivated vaccine carrying the HA and NA segments of A/HK/156/97 (H5N1) that was both rescued from and propagated on PER.C6 cells, conferred protection in a mouse model. Thus PER.C6 cells provide an attractive platform for generation of influenza vaccine strains via reverse genetics. © 2009 Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
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