Indomethacin attenuates mechanical allodynia during the organization but not the maintenance of the peripheral neuropathic pain induced by nervus ischiadicus chronic constriction injury
| Autor: | I.R. dos Santos, Patrícia Medeiros, Norberto Cysne Coimbra, R.L. de Freitas, J.A. da Silva, S H Ferreira, Ana Carolina Medeiros |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Medicine (General) Physiology medicine.medical_treatment Indomethacin Neuropathic pain Biochemistry Rats Sprague-Dawley 0302 clinical medicine Medicine Biology (General) General Pharmacology Toxicology and Pharmaceutics Pain Measurement biology General Neuroscience Sham surgery General Medicine Constriction Sciatic Nerve Peripheral NERVO CIÁTICO 030220 oncology & carcinogenesis Anesthesia von Frey test Non-steroidal anti-inflammatory drugs Research Article Pain Threshold QH301-705.5 Immunology Biophysics Ocean Engineering Mechanical Allodynia 03 medical and health sciences R5-920 Neurochemical Mechanical allodynia Chronic constriction injury of the nervus ischiadicus Animals Nociception assay Rats Wistar Ligature business.industry Cell Biology Rats Disease Models Animal 030104 developmental biology biology.protein Neuralgia Cyclooxygenase business |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Medical and Biological Research v.53 n.5 2020 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Vol 53, Iss 5 (2020) |
| Popis: | The neurochemical mechanisms underlying neuropathic pain (NP) are related to peripheral and central sensitization caused by the release of inflammatory mediators in the peripheral damaged tissue and ectopic discharges from the injured nerve, leading to a hyperexcitable state of spinal dorsal horn neurons. The aim of this work was to clarify the role played by cyclooxygenase (COX) in the lesioned peripheral nerve in the development and maintenance of NP by evaluating at which moment the non-steroidal anti-inflammatory drug indomethacin, a non-selective COX inhibitor, attenuated mechanical allodynia after placing one loose ligature around the nervus ischiadicus, an adaptation of Bennett and Xie's model in rodents. NP was induced in male Wistar rats by subjecting them to chronic constriction injury (CCI) of the nervus ischiadicus, placing one loose ligature around the peripheral nerve, and a sham surgery (without CCI) was used as control. Indomethacin (2 mg/kg) or vehicle was intraperitoneally and acutely administered in each group of rats and at different time windows (1, 2, 4, 7, 14, 21, and 28 days) after the CCI or sham surgical procedures, followed by von Frey's test for 30 min. The data showed that indomethacin decreased the mechanical allodynia threshold of rats on the first, second, and fourth days after CCI (P |
| Databáze: | OpenAIRE |
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