Persistence of probiotic strains in the gastrointestinal tract when administered as capsules, yoghurt, or cheese
| Autor: | Riitta Korpela, Salme Järvenpää, Heikki Vapaatalo, Anu Surakka, Katja Hatakka, Maija Saxelin, Soile Tynkkynen, Marja Mutanen, Anna Lassig, Heli Karjalainen |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Adolescent Propionibacterium Capsules Microbiology law.invention 03 medical and health sciences Probiotic Feces Young Adult fluids and secretions Lactobacillus rhamnosus law Cheese Lactobacillus Humans Food science Saliva 030304 developmental biology Bifidobacterium 0303 health sciences biology 030306 microbiology Propionibacterium freudenreichii Lacticaseibacillus rhamnosus Probiotics food and beverages General Medicine Middle Aged biology.organism_classification Yogurt Bifidobacterium animalis Bifidobacteriaceae Gastrointestinal Tract Female Food Science |
| Zdroj: | International journal of food microbiology. 144(2) |
| ISSN: | 1879-3460 |
| Popis: | Most clinical studies of probiotics use freeze-dried, powdered bacteria or bacteria packed in capsules. However, probiotics are commercially available in various food matrices, which may affect their persistence in the gastrointestinal tract. The objective of the study was to compare oral and faecal recovery during and after administration of a combination of Lactobacillus rhamnosus GG and LC705, Propionibacterium freudenreichii subsp. shermanii JS, and Bifidobacterium animalis subsp. lactis Bb12 as capsules, yoghurt, or cheese. This randomized, parallel-group, open-label trial (n=36) included a 4-week run-in, 2-week intervention, and 3-week follow-up period. Participants consumed 10(10)cfu/day of probiotic combination and provided saliva and faecal samples before, during, and after the intervention. Strain-specific real-time PCR was used to quantify the strains. L. rhamnosus GG was the only probiotic strain regularly recovered in saliva samples. During the intervention period it was recovered in the saliva of 88% of the volunteers at least once. No difference was found between the yoghurt and cheese groups. At the end of the intervention, L. rhamnosus GG and LC705 counts were high in faecal samples of all product groups (8.08 and 8.67log(10) genome copies/g, respectively). There was no matrix effect on strain quantity in faeces or the recovery time after ceasing the intervention. For P. freudenreichii subsp. shermanii JS and B. animalis subsp. lactis Bb12, a matrix effect was found at the end of the intervention (P |
| Databáze: | OpenAIRE |
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