Effects of irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA and induction of apoptosis in HeLa cells and nasal mucosa of rats
| Autor: | Yoshiaki Kitamura, Nanae Sanada, Shotaro Michinaga, Noriaki Takeda, Kentaro Okamoto, Takuya Yamada, Mika Kitayama, Manabu Sugiyama, Hiroyuki Mizuguchi, Tatsuya Fujii, Hiroyuki Fukui |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pharmacology integumentary system medicine.diagnostic_test biology Chemistry lcsh:RM1-950 Mucous membrane of nose Histamine H1 receptor biology.organism_classification Molecular biology HeLa 030207 dermatology & venereal diseases 03 medical and health sciences lcsh:Therapeutics. Pharmacology 030104 developmental biology 0302 clinical medicine Downregulation and upregulation Western blot Apoptosis Gene expression medicine Molecular Medicine Nasal administration |
| Zdroj: | Journal of Pharmacological Sciences, Vol 138, Iss 1, Pp 54-62 (2018) |
| ISSN: | 1347-8613 |
| Popis: | Narrowband-ultraviolet B (NB-UVB) phototherapy is used for the treatment of atopic dermatitis. Previously, we reported that irradiation with 200 mJ/cm2 of 310 nm NB-UVB suppressed phorbol-12-myristate-13-acetate (PMA)-induced up-regulation of histamine H1 receptor (H1R) gene expression without induction of apoptosis in HeLa cells. However, the effect of NB-UVB irradiation on nasal symptoms is still unclear. Here, we show that low dose irradiation with 310 nm NB-UVB alleviates nasal symptoms in toluene 2,4-diisocyanate (TDI)-sensitized allergy model rats. Irradiation with 310 nm NB-UVB suppressed PMA-induced H1R mRNA up-regulation in HeLa cells dose-dependently at doses of 75–200 mJ/cm2 and reversibly at a dose of 150 mJ/cm2 without induction of apoptosis. While, at doses of more than 200 mJ/cm2, irradiation with 310 nm NB-UVB induced apoptosis. Western blot analysis showed that the suppressive effect of NB-UVB irradiation on H1R gene expression was through the inhibition of ERK phosphorylation. In TDI-sensitized rat, intranasal irradiation with 310 nm NB-UVB at an estimated dose of 100 mJ/cm2 once a day for three days suppressed TDI-induced sneezes and up-regulation of H1R mRNA in nasal mucosa without induction of apoptosis. These findings suggest that repeated intranasal irradiation with low dose of NB-UVB could be clinically used as phototherapy of AR. Keywords: Allergic rhinitis, Apoptosis, Histamine H1 receptor gene, Narrowband-ultraviolet B, Phototherapy |
| Databáze: | OpenAIRE |
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