Proteomic analysis of Lactobacillus pentosus for the identification of potential markers involved in acid resistance and their influence on other probiotic features

Autor: Hikmate Abriouel, Saïd Ennahar, Peter Horvatovich, Nabil Benomar, Charles W. Knapp, Natacha Caballero Gómez, Beatriz Pérez Montoro, Antonio Gálvez
Přispěvatelé: Analytical Biochemistry, Medicinal Chemistry and Bioanalysis (MCB), Area de Microbiologıia, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaén (UJA), Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Dpt Civil, Environmental & Architectural Engineering, University of Kansas, University of Kansas [Lawrence] (KU)
Rok vydání: 2018
Předmět:
Zdroj: Food Microbiology, 72(Supplement C), 31-38. ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD
Food Microbiology
Food Microbiology, Elsevier, 2018, 72, pp.31-38. ⟨10.1016/j.fm.2017.11.006⟩
ISSN: 0740-0020
1095-9998
DOI: 10.1016/j.fm.2017.11.006
Popis: Acidity often prevents the undesirable microbial colonization both in fermented foods and under gastric conditions. Thus, the acid resistance of Lactobacillus pentosus strains used as starter cultures and/or probiotics requires further understanding. This was investigated by means of comparative proteomic approach using three strains representing the phenotypes: resistant (AP2-15), intermediate (AP2-18) and sensitive (LP-1) to acidic conditions. Proteomic analysis of constitutive phenotypes revealed that the intrinsic resistance of L. pentosus is associated with the over-production of three principal proteins: 2,3-bisphosphoglycerate- dependent phosphoglycerate mutase 2 (PGAM-d), elongation factor G and 50S ribosomal protein L10, and additionally on ATP synthase subunit beta and chaperone protein DnaK; they are associated with metabolic pathways of proteins and carbohydrates, energy production and stress responses. Suggested protein biomarkers for acid resistance in L. pentosus include elongation factor G and PGAM-d, both being abundantly found in the constitutive proteome of the resistant phenotype under standard and acidic conditions. Furthermore, L. pentosus strains pre-exposed to acids displayed enhanced probiotic function such as auto-aggregation ability via surface proteins. We conclude that pre-exposure of probiotic L. pentosus strains to acid may strategically enhance their performance as starter cultures and probiotics. (c) 2017 Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
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